WISP-1/CCN4 Antibody (213609) [Alexa Fluor® 647]

• Applications: ELISA(Cap), ELISA(Det)
• Clone: 213609
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

WISP-1/CCN4 Antibody (213609) [Alexa Fluor® 647] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human WISP-1/CCN4
  Thr23-Asn367
  Accession # O95388
• Specificity: Detects human WISP-1 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) Biglycan, rhDecorin, rhNOV, or rhWISP-3 was observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Human WISP-1 (Wnt-induced secreted protein-1; also CNN4) is a 40 kDa, secreted, heparin-binding glycoprotein that is a member of the CCN (or CTGF/Cyr61/Nov) cysteine-rich protein family (1 - 5).  It is synthesized as a 367 aa precursor that contains a series of structural homology modules.  Following a 22 amino acid (aa) signal sequence, there is a 68 aa IGFBP-like domain (aa 53 - 120), a 57 aa von Willebrand factor type C (VWC) module (aa 126 - 182), a 40 aa TSP type I domain (aa 220 - 259) and a 75 aa, C-terminal cysteine knot motif (aa 273 - 347).  The VWC module is associated with protein-protein interaction, the TSP domain binds sulfated glycoconjugates, and the cysteine knot mediates dimerization and receptor binding (4).  It is likely that WISP-1 normally circulates as an 80 kDa homodimer (2).  At least five alternative splice forms are known for WISP-1.  One is 30 kDa in size, 258 aa in length, and shows a substitution of a His for aa 95 - 182.  This removes the VWC domain (2, 6).  A second isoform is 155 aa in length and shows a frameshift at Arg 117 with a unique 38 aa C-terminal extension.  A third is 195 aa in length and shows a 31 aa substitution for the first 203 aa of the full length precursor (6).  This retains the VWC and cysteine knot domains.  A fourth shows a 43 aa substitution for aa 117 - 367 for a total length of 163 aa.  This effectively removes everything but the IGFBP-like domain (7).  The last splice form contains a deletion of aa 25 - 269 for a total length of 122 aa.  Thus, only the signal sequence and cysteine knot motifs are retained (8).  This leaves only the IGFBP-like domain (9).  Full-length mature human WISP-1 is 85% aa identical to both mouse and rat WISP-1.  WISP-1 is expressed by osteoblasts and may contribute to fracture healing by promoting bone cell formation (10, 11). 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.