WIPI2 Antibody

Images

 
Western Blot: WIPI2 Antibody [NBP2-20906] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: WIPI2 Antibody [NBP2-20906] - A431 cells were fixed in ice-cold MeOH for 5 min. Green: WIPI2 protein stained by WIPI2 antibody diluted at 1:200. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: WIPI2 Antibody [NBP2-20906] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.
Immunocytochemistry/ Immunofluorescence: WIPI2 Antibody [NBP2-20906] - Immunofluorescence analysis of methanol-fixed A431, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

WIPI2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human WIPI2. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WIPI2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for WIPI2 Antibody

  • ATG18B
  • Atg21
  • CGI-50
  • DKFZP434J154
  • DKFZp686P02188
  • FLJ12979
  • FLJ14217
  • FLJ42984
  • WD repeat domain phosphoinositide-interacting protein 2
  • WD repeat domain, phosphoinositide interacting 2
  • WD40 repeat protein interacting with phosphoinositides 2
  • WIPI-2
  • WIPI49-like protein 2

Background

WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol WIPI2
Uniprot