WDR74 Antibody - BSA Free

Western Blot: WDR74 Antibody [NBP1-77140] - Mouse liver tissue lysate with NSA1 antibody at (A) 1 and (B) 2 ug/mL.

Immunohistochemistry: WDR74 Antibody - BSA Free [NBP1-77140] - Immunohistochemistry of WDR74 in rat liver tissue with WDR74 antibody at 5 ug/mL.

Immunocytochemistry/ Immunofluorescence: WDR74 Antibody - BSA Free [NBP1-77140] - Immunofluorescence of WDR74 in rat liver tissue with WDR74 antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

WDR74 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit WDR74 Antibody - BSA Free (NBP1-77140) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human NSA1. The immunogen is located within amino acids 320 - 370 of NSA1. Amino Acid Squence: EPQEPNKVPLEDTETDEL
• Predicted Species: Bovine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WDR74
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1-2 ug/ml
• Control Peptide:

  WDR74 Peptide (NBP1-77140PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for WDR74 Antibody - BSA Free

• FLJ10439
• FLJ21730
• NOP seven-associated protein 1
• NSA1
• WD repeat domain 74
• WD repeat-containing protein 74

Background

The yeast nucleolar protein NOP7 is necessary for the maturation of 66S preribosomes and interacts with numerous other proteins. One such protein is an essential, conserved WD repeat protein, NOP seven-associated protein 1 (NSA1), that is also required for the yeast 66S ribosome assembly. NSA1 is also associated with the AAA ATPase Rix7, and release of NSA1 from a novel late nucleolar pre-60S requires the Rix7 function. NSA1 has also been found upregulated in mammalian cancer cells, suggesting it may also play a role in cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WDR74
• Uniprot:
  • Q6RFH5(Human)