SMOC-2 Antibody (667713)

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SMOC-2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human SMOC-2 Monoclonal Antibody (Catalog # MAB5140) at 15 µg/mL overnight at 4 °C. Before incubation with the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
667713
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

SMOC-2 Antibody (667713) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human SMOC-2
Gln22-Gly446
Accession # Q9H3U7
Specificity
Detects human SMOC-2 in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human (rh) SMOC-1 and no cross-reactivity with recombinant mouse SMOC-2 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SMOC2
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
Publications
Read Publication using
MAB5140 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SMOC-2 Antibody (667713)

  • bA270C4A.1
  • bA37D8.1
  • dJ421D16.1
  • MST117
  • MSTP117
  • MSTP140
  • SMAP2
  • SMAP-2
  • SMAP2Secreted modular calcium-binding protein 2
  • SMOC2
  • SMOC-2
  • smooth muscle associated protein 2
  • Smooth muscle-associated protein 2
  • SPARC related modular calcium binding 2
  • SPARC-related modular calcium-binding protein 2
  • thyroglobulin type-1 repeat containing protein

Background

SMOC-2 (secreted modular calcium-binding protein 2; also SMAP2) is a 55 kDa glycoprotein member of the SPARC family of matricellular proteins.  It is widely expressed, perhaps by endothelium, and apparently participates in both growth factor signaling and ECM organization.  Mature human SMOC-2 is 425 amino acids (aa) in length.  It contains one Kazal-like domain (aa 40-84), two thyroglobulin type-1 segments (aa 87-153 and 213-281), and two EF-hand sequences (aa 347-382 and 384‑419).  There are three splice variants.  One shows a 13 aa substitution for aa 442-446, another shows an 11 aa insertion after Thr170, and a third represents a combination of the above two variations.  Over aa 22-446, human SMOC-2 shares 95% aa identity with mouse SMOC-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SMOC-2 Antibody (MAB5140)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Functional Assay.


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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SMOC2
Entrez
Uniprot