WDR44 Antibody

Western Blot: WDR44 Antibody [NB100-68250] - WDR44 was silenced with shRNA lentivirus in HeLa cells. WDR44 was evaluated by immunoblot in cells treated with shRNA control and shRNA WDR44. Image from verified customer review.

Western Blot: WDR44 Antibody [NB100-68250] - Detection of Human WDR44 on HeLa whole cell lysate using NB100-68250. WDR44 was also immunoprecipitated by rabbit anti-WDR44 antibody NB100-68251.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

WDR44 Antibody Summary

• Description: Novus Biologicals Rabbit WDR44 Antibody (NB100-68250) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 100 and 150 of human WD repeat domain 44 using the numbering given in entry NP_061918.3 (GeneID 54521).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WDR44
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Control:

  WDR44 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for WDR44 Antibody

• DKFZp686L20145
• FLJ13230
• MGC26781
• RAB11BP
• rabphilin-11
• RPH11
• WD repeat domain 44
• WD repeat-containing protein 44

Background

WDR44 is a member of the WD repeat family. The WD repeat is defined by four or more repeating units of a conserved core of approximately 40 amino acids ending with tryptophan-aspartic acid (WD). WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation. WD-repeat domain 44 (WDR44) was originally identified as rabphilin-11, a protein isolated from bovine brain extracts and cloned from a rat brain cDNA library. Rabphilin-11 was found to bind GTP-Rab11 and proposed to localize to the Golgi and recycling endosomes suggesting a role in vesicle recycling. WDR44 contains seven WD (tryptophan-aspartate) repeat domains found in a number of proteins that function as adaptor molecules in signal transduction and cytoskeletal organization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for WDR44 Antibody (NB100-68250) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 03/26/2019

Summary

• Application: Western Blot
• Sample Tested: hela cell
• Species: Human
• Lot: lotA1

Bioinformatics

• Gene Symbol: WDR44
• Entrez:
  • 54521(Human)
• Uniprot:
  • Q5JSH3(Human)