![Immunocytochemistry/Immunofluorescence: WASHC1 Antibody [NBP1-90464] - Staining of human cell line U-251MG shows positivity in vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/WASHC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90464-img0008.jpg "Immunocytochemistry/Immunofluorescence: WASHC1 Antibody [NBP1-90464] - Staining of human cell line U-251MG shows positivity in vesicles. Antibody staining is shown in green.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, Mycoplasma |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit WASHC1 Antibody - BSA Free (NBP1-90464) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-WASHC1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: GAPREVVDPSGGWATLLESIRQAGGIGKAKLRSMKERKLEKKKQKEQEQVRATSQGGHLMSDLFNKLVMRHKGISGKGPGAGEGPGGAFARVSDSIPPLPPPQQPQVDEDEDDWES |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | washc1 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
![Immunohistochemistry EGFR Antibody [Unconjugated]](https://images.novusbio.com/images2/EGF_R_AF231_Immunohistochemistry_20892.jpg)
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| Publication using NBP1-90464 | Applications | Species |
|---|---|---|
| Rahman AA, Soto-Avellaneda A, Yong Jin H et al. Enhanced Hyaluronan Signaling and Autophagy Dysfunction by VPS35 D620N Neuroscience 2020-06-13 [PMID: 32540366] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | washc1 |
![Immunohistochemistry-Paraffin: WASHC1 Antibody [NBP1-90464] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/WASHC1-Antibody-Immunohistochemistry-Paraffin-NBP1-90464-img0013.jpg "Immunohistochemistry-Paraffin: WASHC1 Antibody [NBP1-90464] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: WASHC1 Antibody [NBP1-90464] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/WASHC1-Antibody-Immunohistochemistry-Paraffin-NBP1-90464-img0010.jpg "Immunohistochemistry-Paraffin: WASHC1 Antibody [NBP1-90464] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.")
![Immunohistochemistry-Paraffin: WASHC1 Antibody [NBP1-90464] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/WASHC1-Antibody-Immunohistochemistry-Paraffin-NBP1-90464-img0011.jpg "Immunohistochemistry-Paraffin: WASHC1 Antibody [NBP1-90464] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: WASHC1 Antibody [NBP1-90464] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/WASHC1-Antibody-Immunohistochemistry-Paraffin-NBP1-90464-img0012.jpg "Immunohistochemistry-Paraffin: WASHC1 Antibody [NBP1-90464] - Staining of human liver shows no positivity in hepatocytes as expected.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")