FKBP15 Antibody

Images

 
Western Blot: FKBP15 Antibody [NBP1-77352] - 3T3 cell lysate with FKBP15 antibody at 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: FKBP15 Antibody [NBP1-77352] - Immunofluorescence of FKBP15 in mouse brain tissue with FKBP15 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: FKBP15 Antibody [NBP1-77352] - Mouse brain tissue with FKBP15 antibody at 2.5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

FKBP15 Antibody Summary

Immunogen
Antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human FKBP15. The immunogen is located within amino acids 30 - 80 of FKBP15.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FKBP15
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Control Peptide
FKBP15 Peptide (NBP1-77352PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FKBP15 Antibody

  • FK506 binding protein 15, 133kDa
  • FK506-binding protein 133kDa
  • FK506-binding protein 15
  • FKBP133
  • FKBP-133
  • FKBP-15,133 kDa FKBP
  • PPP1R76,133 kDa FK506-binding protein
  • regulatory subunit 76
  • WAFL

Background

FKBP15, also known as FKBP133, is a member of the FK506-binding protein family, a group of proteins initially identified as immunophilins, targets for the immunosupressant drugs FK506 and Rapamycin. FKBP15 is expressed in the developing nervous system and contains a domain similar to Wiskott-Aldrich syndrome protein homology region 1 (WH1) in addition to the FK506-binding protein motif. FKBP15 is distributed along the axonal shafts and partially co-localizes with F-actin in the growth cones of dorsal root ganglion neurons; overexpression of FKBP15 resulted in the number of filopodia in transfected neurons, suggesting that FKBP15 modulates growth cone behavior. FKBP15 has also been shown to associate with both microtubules and the actin filament systems and disruption of its expression by RNAi resulted in delayed transport of early endosomes in HeLa cells indicating that FKBP15 is also involved in the transport of early endosomes. At least three isoforms of FKBP15 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FKBP15
Uniprot