vWF-A2 Antibody (210909) [Unconjugated]

• Reactivity: Hu
• Applications: WB
• Clone: 210909
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

vWF-A2 Antibody (210909) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human vWF-A2
  Asp1498-Val1665
  Accession # P04275
• Specificity: Detects human vWF-A2 in direct ELISAs and Western blots. This antibody detects an epitope C-terminal to the ADAMTS13 cleavage site between aa 1606-1665.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: VWF
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for vWF-A2 Antibody (210909) [Unconjugated]

• F8VWF
• VWD
• VWF von Willebrand factor
• vWFA2
• vWF-A2

Background

von Willebrand Factor (vWF) is a large, multimeric glycoprotein made by endothelial cells and megakaryocytes. The pre-pro-vWF protein contains 2813 amino acids (aa), which consists of 22 aa signal peptide, 741 aa propeptide, and mature vWF monomer of 2050 aa (1-4). The pro-vWF undergoes dimerization in the endoplasmic reticulum (ER) through C-terminal “cysteine-knot” (CK) domain. The pro-vWF dimmers are transported to Golgi and form multimers by forming disulfide bond in amino-terminal region of the mature form. The proteolytic processing of pro-region also occurs in Golgi. The matured vWF is stored in Weibel-Pallade bodies in endothelial cells and granules in megakaryocytes and platelets. The unusually-large vWF (ulvWF) multimers released from cells are very efficient in binding to platelets to form thrombus. The population of these highly active ulvWF multimers is controlled by a specific protease, ADAMTS13, which cleaves between residues Tyr1605 and Met1606 in the A2 domain of vWF. In the plasma, vWF appears as a series of large and intermediate multimers with molecular masses from several thousand to 500 kDa. vWF also performs hemostatic functions (3-5). In a high shear-stressed environment, vWF undergoes conformational change to expose a binding site for glycoprotein Ib alpha . As a result, vWF facilitates aggregation of platelets. In addition to platelet binding, vWF binds coagulation factor VIII to increase the lifetime of FVIII in plasma. The purified rhvWF-A2 contains the A2 domain of vWF.

1. Sadler, J.E. (1998) Annu. Rev. Biochem. 67:395.
2. Ruggeri, Z.M. (2003) Cur. Opin. Hemat. 10:142.
3. Michiels, J.J. et al. (2006) Clin. Appl. Thromb. Hemost. 12:397.
4. Groot, E. et al. (2007) Cur. Opin. Hemat. 14:284.
5. Lenting, P.J. et al. (2007) J. Thromb. Haemos. 5:1353.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for vWF-A2 Antibody (MAB2764)(3)

Publications using MAB2764

• M Perepitchk, Y Galat, IP Beletsky, PM Iannaccone, V Galat Down syndrome iPSC model: endothelial perspective on tumor development Oncotarget, 2020-09-08;11(36):3387-3404. 2020-09-08 [PMID: 32934781] (ICC, Human)
• D Butera, F Passam, L Ju, KM Cook, H Woon, C Aponte-San, E Gardiner, AK Davis, DA Murphy, A Bronowska, BM Luken, C Baldauf, S Jackson, R Andrews, F Gräter, PJ Hogg Autoregulation of von Willebrand factor function by a disulfide bond switch Sci Adv, 2018-02-28;4(2):eaaq1477. 2018-02-28 [PMID: 29507883] (Binding Assay, Human)
• S Gogia, A Kelkar, C Zhang, KM Dayananda, S Neelamegha Role of calcium in regulating the intra- and extracellular cleavage of von Willebrand factor by the protease ADAMTS13 Blood Adv, 2017-10-20;1(23):2063-2074. 10/20/2017 [PMID: 29296853]

Bioinformatics

• Gene Symbol: VWF
• Uniprot:
  • P04275()