VPS53 Antibody - BSA Free

Western Blot: VPS53 Antibody [NBP2-97767] - Anti-VPS53 rabbit polyclonal antibody at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 80 kDa. Observed band size: 95 kDa

Immunoprecipitation: VPS53 Antibody [NBP2-97767] - VPS53 was immunoprecipitated using: Lane A: 0.5 mg U-251 MG Whole Cell Lysate 4 ul anti-VPS53 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-VPS53 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 80 kDa. Observed band size: 95 kDa

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

VPS53 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human VPS53 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VPS53
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 0.5-2uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for VPS53 Antibody - BSA Free

• FLJ10979
• FLJ41112
• FLJ61757
• HCCS1
• hVps53L
• MGC39512
• pp13624
• vacuolar protein sorting 53 (yeast)
• vacuolar protein sorting 53 homolog (S. cerevisiae)
• vacuolar protein sorting-associated protein 53 homolog

Background

VPS53 encodes a protein with sequence similarity to the yeast Vps53p protein. Vps53p is involved in retrograde vesicle trafficking in late Golgi. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VPS53