VPS35 Antibody - BSA Free

Western Blot: VPS35 Antibody [NBP2-20876] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: VPS35 Antibody [NBP2-20876] - A549 xenograft, using antibody at 1:500 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min

Western Blot: VPS35 Antibody [NBP2-20876] - Sample (30 ug of whole cell lysate) A: HCT116 7. 5% SDS PAGE gel, diluted at 1:1000.

Western Blot: VPS35 Antibody [NBP2-20876] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunoprecipitation: VPS35 Antibody [NBP2-20876] - Immunoprecipitation of VPS35 protein from HeLa whole cell extracts using 5 ug of VPS35 antibody. Western blot analysis was performed using VPS35 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

• Reactivity: Hu, Mu, Rt, Bv, Ch, Xp, Ze
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

VPS35 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit VPS35 Antibody - BSA Free (NBP2-20876) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-VPS35 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human VPS35. The exact sequence is proprietary.
• Localization: Cytoplasm; Membrane; Peripheral membrane protein
• Predicted Species: Rat (100%), Chicken (98%), Xenopus (97%), Bovine (100%), Zebrafish (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VPS35
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 92 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (97%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for VPS35 Antibody - BSA Free

• DKFZp434P1672
• FLJ10752
• FLJ13588
• FLJ20388
• hVPS35
• Maternal-embryonic 3
• MEM3DKFZp434E1211
• vacuolar protein sorting 35 (yeast homolog)
• vacuolar protein sorting 35 (yeast)
• vacuolar protein sorting 35 homolog (S. cerevisiae)
• vacuolar protein sorting-associated protein 35
• Vesicle protein sorting 35

Background

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for VPS35 Antibody (NBP2-20876)(1)

Publication using NBP2-20876

• Yin J, Liu X, He Q et al. Vps35-dependent recycling of Trem2 regulates microglial function. Traffic 2016-09-26 [PMID: 27717139]

Bioinformatics

• Gene Symbol: VPS35
• Uniprot:
  • Q96QK1(Human)