VPS35 Antibody (HL1017) - Azide and BSA Free

Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: VPS35 Antibody (HL1017) [NBP3-13680] - VPS35 antibody detects VPS35 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS35 stained by VPS35 antibody (NBP3-13680) diluted at 1:500.

Immunohistochemistry-Paraffin: VPS35 Antibody (HL1017) [NBP3-13680] - VPS35 antibody [HL1017] detects VPS35 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. VPS35 stained by VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Whole zebrafish extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemistry-Paraffin: VPS35 Antibody (HL1017) [NBP3-13680] - VPS35 antibody [HL1017] detects VPS35 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissue.VPS35 stained by VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: VPS35 Antibody (HL1017) [NBP3-13680] - VPS35 antibody [HL1017] detects VPS35 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat testis.VPS35 stained by VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: HL1017
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

VPS35 Antibody (HL1017) - Azide and BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VPS35. The exact sequence is proprietary.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: VPS35
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for VPS35 Antibody (HL1017) - Azide and BSA Free

• DKFZp434P1672
• FLJ10752
• FLJ13588
• FLJ20388
• hVPS35
• Maternal-embryonic 3
• MEM3DKFZp434E1211
• vacuolar protein sorting 35 (yeast homolog)
• vacuolar protein sorting 35 (yeast)
• vacuolar protein sorting 35 homolog (S. cerevisiae)
• vacuolar protein sorting-associated protein 35
• Vesicle protein sorting 35

Background

The maintenance of organelles in eukaryotic cells depends on sorting proteins, which ensure the proper delivery of organelle-specific proteins. In S. cerevisiae, VPS35 has a direct role in the retrieval of vacuolar cargo proteins, suggesting that human VPS35 may have an analogous function in the maintenance of lysosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VPS35