VPS28 Antibody

Western Blot: VPS28 Antibody [NB100-1399] - (1ug/ml) of Human Testis lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Immunohistochemistry-Paraffin: VPS28 Antibody [NB100-1399] - (10ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

• Reactivity: Hu, Mu, Rt, Po, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

VPS28 Antibody Summary

• Immunogen: Peptide with sequence C-ESAYNAFNRFLHA corresponding to C-Terminus according to NP_057292.1.
• Specificity: This antibody is expected to recognize isoform 1 (NP_057292.1) only.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: VPS28
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 24 kDa band observed in H460, human testis and brain lysates (predicted MW of 25 kDa band according to NP_057292.1). IHC-P: Human testis stains acrosomal head caps in round spermatids.
• Control:

  Human Testis Whole Tissue Lysate (Adult Whole Normal)
  Human Brain Whole Tissue Lysate (Adult Whole Normal)
  VPS28 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for VPS28 Antibody

• ESCRT-I complex subunit VPS28
• H-Vps28
• MGC60323
• vacuolar protein sorting 28 (yeast)
• vacuolar protein sorting 28 homolog (S. cerevisiae)
• vacuolar protein sorting-associated protein 28 homolog
• yeast class E protein Vps28p homolog

Background

This gene encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. The two other subunits of ESCRT-I are vesicular protein sorting 23, also known as tumor susceptibility gene 101 (TSG101), and vesicular protein sorting 37. Two alternative transcripts encoding different isoforms have been described. Additional alternative transcripts may exist but the proteins encoded by these transcripts have not been verified experimentally.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VPS28 Antibody (NB100-1399)(1)

Publication using NB100-1399

• Bishop N, Woodman P. TSG101/mammalian VPS23 and mammalian VPS28 interact directly and are recruited to VPS4-induced endosomes. J Biol Chem 2001-04-13 [PMID: 11134028]

Bioinformatics

• Gene Symbol: VPS28
• Entrez:
  • 51160(Human)
  • 66914(Mouse)
• Uniprot:
  • NP_057292.(Human)