VPS28 Antibody (OTI1A8) - Azide and BSA Free

Images

 
Western Blot: VPS28 Antibody (OTI1A8) - Azide and BSA Free [NBP2-74854] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VPS28.
Immunocytochemistry/ Immunofluorescence: VPS28 Antibody (OTI1A8) - Azide and BSA Free [NBP2-74854] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY VPS28.
Immunohistochemistry: VPS28 Antibody (OTI1A8) - Azide and BSA Free [NBP2-74854] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: VPS28 Antibody (OTI1A8) - Azide and BSA Free [NBP2-74854] - Analysis of extracts (35ug) from 9 different cell line (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI1A8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

VPS28 Antibody (OTI1A8) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human VPS28(NP_057292) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
VPS28
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:200-2000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
Theoretical MW
25.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for VPS28 Antibody (OTI1A8) - Azide and BSA Free

  • ESCRT-I complex subunit VPS28
  • H-Vps28
  • MGC60323
  • vacuolar protein sorting 28 (yeast)
  • vacuolar protein sorting 28 homolog (S. cerevisiae)
  • vacuolar protein sorting-associated protein 28 homolog
  • yeast class E protein Vps28p homolog

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for VPS28 Antibody (NBP2-74854) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-74854AF350
Alexa Fluor 405 NBP2-74854AF405
Alexa Fluor 488 NBP2-74854AF488
Alexa Fluor 532 NBP2-74854AF532
Alexa Fluor 594 NBP2-74854AF594
Alexa Fluor 647 NBP2-74854AF647
Alexa Fluor 700 NBP2-74854AF700
Alexa Fluor 750 NBP2-74854AF750
Biotin NBP2-74854B
DyLight 350 NBP2-74854UV
DyLight 405 NBP2-74854V
DyLight 488 NBP2-74854G
DyLight 550 NBP2-74854R
DyLight 594 NBP2-74854DL594
DyLight 650 NBP2-74854C
DyLight 680 NBP2-74854FR
DyLight 755 NBP2-74854IR
FITC NBP2-74854F
HRP NBP2-74854H
Janelia Fluor 549 NBP2-74854JF549
Janelia Fluor 646 NBP2-74854JF646

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Calculators

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Bioinformatics

Gene Symbol VPS28