VPS26A Antibody (CL2287) - Azide and BSA Free

Staining in U251 cell line with Anti-VSP26A monoclonal antibody) .

Staining in MCF7 cell line with Anti-VSP26A monoclonal antibody) .

Analysis in human testis and skeletal muscle tissues using NBP3-44464 antibody. Corresponding VPS26A RNA-seq data are presented for the same tissues.

Lane 1: Marker [kDa]Lane 2: Human cell line U-251 MG

Staining in HeLa cell line with Anti-VSP26A monoclonal antibody) .

Staining in A431 cell line with Anti-VSP26A monoclonal antibody) .

Staining in U2OS cell line with Anti-VSP26A monoclonal antibody) .

Staining of human duodenum shows moderate to strong cytoplasmic positivity in glandular cells.

Staining of human fallopian tube shows moderate to strong cytoplasmic positivity in glandular cells.

Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.

Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.

Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-VPS26A antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL2287
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

VPS26A Antibody (CL2287) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a recombinant protein sequence of O75436, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: VPS26A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for VPS26A Antibody (CL2287) - Azide and BSA Free

• FLJ12930
• HB58
• Hbeta58
• hVPS26
• PEP8A
• vacuolar protein sorting 26 (yeast homolog)
• vacuolar protein sorting 26 homolog A (S. pombe)
• vacuolar protein sorting 26 homolog A (yeast)
• vacuolar protein sorting-associated protein 26A
• Vesicle protein sorting 26A
• VPS26vacuolar protein sorting 26 (yeast)

Background

VPS26 belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VPS26A