Recombinant Human VOPP1 His Protein

SDS-Page: Recombinant Human VOPP1 His Protein [NBP1-72322] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human VOPP1 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 82-172 of Human VOPP1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MRRRMYPPPL IEEPAFNVSY TRQPPNPGPG AQQPGPPYYT DPGGPGMNPV GNSMAMAFQV PPNSPQGSVA CPPPPAYCNT PPPPYEQVVK AK

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: VOPP1
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 12 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 30% glycerol, 0.2 M NaCl
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human VOPP1 His Protein

• ECop
• ECOPDKFZp564K0822
• EGFR-coamplified and overexpressed protein
• FLJ20532
• GASPGlioblastoma-amplified secreted protein
• Putative NF-kappa-B-activating protein 055N
• vesicular, overexpressed in cancer, prosurvival protein 1

Background

Vesicular, overexpressed in cancer, prosurvival protein 1(VOPP1), also known as Glioblastoma-amplified secreted protein (GASP), has been previously shown to be over-expressed in human glioblastoma multiforme and squamous cell carcinoma. VOPP1 is a key regulator of NF-kappaB signaling, and that high-level, amplification-mediated VOPP1 expression, such as that occurring in tumors with amplified EGFR, could contribute to resistance to apoptosis. Recombinant human VOPP1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: VOPP1