VMAT2 Antibody - BSA Free

• Reactivity: Hu, Mu, Rt, Rb
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

VMAT2 Antibody - BSA Free Summary

• Immunogen: Reacts with an internal sequence of the human, mouse and rat VMAT2 protein.
• Specificity: VMAT2
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC18A2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:100-1:250
  • Immunohistochemistry-Frozen 1:100-1:250
  • Western Blot 1:500-1:1000
• Application Notes: This antibody is useful for Western Blot. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 20815037). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 24161354). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 55.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Reacts with human, mouse, and rat VMAT2 protein. Rabbit reactivity reported in scientific literature (PMID: 20665056)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for VMAT2 Antibody - BSA Free

• MGC120477
• MNAT
• monoamine neurotransmitter transporter
• Monoamine transporter
• SLC18A2
• solute carrier family 18 (vesicular monoamine), member 2
• Solute carrier family 18 member 2
• SVAT
• SVMT
• SVMTMGC120478
• synaptic vesicular amine transporter
• VAT2
• VAT2MGC26538
• vesicle monoamine transporter type 2
• vesicle monoamine/H+ antiporter
• Vesicular amine transporter 2
• VMAT2

Background

VMAT2 is involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. It is a requisite for vesicular amine storage prior to secretion via exocytosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VMAT2 Antibody (NB110-68123)(10)

Publications using NB110-68123

• Cao SX, Wen CX, Sun R et al. ErbB4 regulate extracellular dopamine through the p38 MAPK signaling pathway Neuroscience letters 2021-03-17 [PMID: 33722543] (WB)
• Jung, D H, Ahn, S M Et al. Therapeutic effects of anodal transcranial direct current stimulation in a rat model of ADHD. Elife 2020-09-21 [PMID: 32955434] (IF/IHC, Mouse)
• Ma, K;Han, C;Zhang, G;Guo, X;Xia, Y;Wan, F;Yin, S;Kou, L;Liu, L;Huang, J;Xiong, N;Wang, T; Reduced VMAT2 expression exacerbates the hyposmia in the MPTP model of Parkinson's disease Biochem. Biophys. Res. Commun. 2019-05-28 [PMID: 30954223] (WB, Mouse)
• Gemechu JM, Sharma A, Yu D et al. Characterization of Dopaminergic System in the Striatum of Young Adult Park2-/- Knockout Rats Sci Rep 2018-01-24 [PMID: 29367643] (WB, Rat)
• Lin CC, Tung CS, Lin PH et al. Traumatic stress causes distinctive effects on fear circuit catecholamines and the fear extinction profile in a rodent model of posttraumatic stress disorder. Eur Neuropsychopharmacol. 2016-09-01 [PMID: 27492886] (WB, Rat)
• Choi W, Kim H, Xia Z. JNK inhibition of VMAT2 contributes to rotenone-induced oxidative stress and dopamine neuron death. Toxicology. 2014-12-09 [PMID: 25496994] (WB)
• Hong F, Liu L, Fan RF et al. New perspectives of vesicular monoamine transporter 2 chemical characteristics in mammals and its constant expression in type 1 diabetes rat models. Transl Res. 2013-10-06 [PMID: 24161354] (ICC/IF, IHC-Fr, Rat)
• Shin EJ, Duong CX, Nguyen XK, Li Z, Bing G, Bach JH, Park DH, Nakayama K, Ali SF, Kanthasamy AG, Cadet JL, Nabeshima T, Kim HC. Role of oxidative stress in methamphetamine-induced dopaminergic toxicity mediated by protein kinase C delta. Behav Brain Res;232(1):98-113. 2012-04-09 [PMID: 22512859] (WB, Mouse)
• Iritani S, Sekiguchi H, Habuchi C et al. Immunohistochemical study of vesicle monoamine transporter 2 in the hippocampal region of genetic animal model of schizophrenia. Synapse;64(12):948-53. 2010-12-01 [PMID: 20815037] (IHC-Fr, Mouse)
• Sala G, Brighina L, Saracchi E et al. Vesicular monoamine transporter 2 mRNA levels are reduced in platelets from patients with Parkinson's disease. J Neural Transm. 2010-09-01 [PMID: 20665056] (WB, Rabbit)

Bioinformatics

• Gene Symbol: SLC18A2
• Entrez:
  • 6571(Human)
  • 214084(Mouse)
  • 25549(Rat)
• Uniprot:
  • Q05940()
  • Q8BRU6(Mouse)
  • Q01827(Rat)