VMAT2 Antibody (OTI9E11) - Azide and BSA Free

Western Blot: VMAT2 Antibody (OTI9E11) - Azide and BSA Free [NBP2-74850] - VMAT2 protein level by using anti-VMAT2 (9E11) antibody in the striatum of autopsied human brain.

Immunocytochemistry/Immunofluorescence: VMAT2 Antibody (OTI9E11) - Azide and BSA Free [NBP2-74850] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY VMAT2.

Immunohistochemistry: VMAT2 Antibody (OTI9E11) - Azide and BSA Free [NBP2-74850] - Staining of paraffin-embedded Human pancreas tissue using anti-VMAT2 mouse monoclonal antibody.

Flow Cytometry: VMAT2 Antibody (OTI9E11) - Azide and BSA Free [NBP2-74850] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-VMAT2 antibody, and then analyzed by flow cytometry.

Western Blot: VMAT2 Antibody (OTI9E11) - Azide and BSA Free [NBP2-74850] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti SLC18A2 monoclonal antibody.(1:200).

Western Blot: VMAT2 Antibody (OTI9E11) - Azide and BSA Free [NBP2-74850] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VMAT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VMAT2.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI9E11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

VMAT2 Antibody (OTI9E11) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse VMAT2 Antibody (OTI9E11) - Azide and BSA Free (NBP1-47977) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full-length protein expressed in 293T cell transfected with human SLC18A2 expression vector .
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC18A2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2000
• Theoretical MW: 55.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for VMAT2 Antibody (OTI9E11) - Azide and BSA Free

• MGC120477
• MNAT
• monoamine neurotransmitter transporter
• Monoamine transporter
• SLC18A2
• solute carrier family 18 (vesicular monoamine), member 2
• Solute carrier family 18 member 2
• SVAT
• SVMT
• SVMTMGC120478
• synaptic vesicular amine transporter
• VAT2
• VAT2MGC26538
• vesicle monoamine transporter type 2
• vesicle monoamine/H+ antiporter
• Vesicular amine transporter 2
• VMAT2

Background

VMAT2 (Vesicular amine transporter 2) acts to accumulate cytosolic monoamines into synaptic vesicles. Its function is essential to the correct activity of the monoaminergic systems that have been implicated in several human neuropsychiatric disorders such as dementia with Lewy bodies. The transporter is a site of action of important drugs, including reserpine and tetrabenazine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC18A2