VMAT2 Antibody (899327) [Unconjugated]

Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human VMAT2. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human VMAT2 Monoclonal Antibody (Catalog # MAB8327) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for VMAT2 at approximately 75-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

HEK293 human embryonic kidney cell line transfected with human VMAT2 and eGFP was stained with either (A) Mouse Anti-Human VMAT2 Monoclonal Antibody (Catalog # MAB8327) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

VMAT2 was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Mouse Anti-Human VMAT2 Monoclonal Antibody (Catalog # MAB8327) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: 899327
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

VMAT2 Antibody (899327) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human VMAT2
  Met1-Asp514
  Accession # Q05940
• Specificity: Detects human VMAT2 in ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC18A2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VMAT2 Antibody (899327) [Unconjugated]

• MGC120477
• MNAT
• monoamine neurotransmitter transporter
• Monoamine transporter
• SLC18A2
• solute carrier family 18 (vesicular monoamine), member 2
• Solute carrier family 18 member 2
• SVAT
• SVMT
• SVMTMGC120478
• synaptic vesicular amine transporter
• VAT2
• VAT2MGC26538
• vesicle monoamine transporter type 2
• vesicle monoamine/H+ antiporter
• Vesicular amine transporter 2
• VMAT2

Background

The Vesicular Monoamine Transporter 2 (VMAT2), also known as VAT2 and SCL18A, is a 55-75 kDa member of the vesicular transporter family, a major facilitator superfamily. VMAT2 is a 12 transmembrane (TM) glycoprotein that is found in the membrane of brain neurosecretory vesicles. It transports monoamines (dopamine, serotonin, and particularly histamine) from the cytosol into secretion vesicles by exchanging two H⁺ ions for one molecule of amine. Human VMAT2 is 514 amino acids (aa) in length. It contains two cytoplasmic domains, a 20 aa and a 52 aa N- and C-terminal respectively, plus an extended 88 aa luminal loop between aa 42-129. There is one luminal, intrachain disulfide bond that contributes to amine transport (C126-C333). In addition, residues in TM domains 5-8 (aa 220-352) and 9-12 (aa 358‑462) also contribute to high affinity ligand interaction. VMAT2 is constitutively phosphorylated by CKII on S511 and S513. Within the cytoplasmic C-terminus, human VMAT2 is 94% aa identical to rat VMAT2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VMAT2 Antibody (MAB8327)(1)

Publication using MAB8327

• Victoria C. de Leeuw, Conny T. M. van Oostrom, Edwin P. Zwart, Harm J. Heusinkveld, Ellen V. S. Hessel Prolonged Differentiation of Neuron-Astrocyte Co-Cultures Results in Emergence of Dopaminergic Neurons International Journal of Molecular Sciences 2023-02-10 [PMID: 36835019]

Bioinformatics

• Gene Symbol: SLC18A2
• Uniprot:
  • Q05940()