VMAT2 Antibody (899327) [Alexa Fluor™ Plus 647]

• Clone: 899327
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 647

VMAT2 Antibody (899327) [Alexa Fluor™ Plus 647] Summary

• Specificity: Detects human VMAT2 in ELISAs and Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

The Vesicular Monoamine Transporter 2 (VMAT2), also known as VAT2 and SCL18A, is a 55-75 kDa member of the vesicular transporter family, a major facilitator superfamily. VMAT2 is a 12 transmembrane (TM) glycoprotein that is found in the membrane of brain neurosecretory vesicles. It transports monoamines (dopamine, serotonin, and particularly histamine) from the cytosol into secretion vesicles by exchanging two H⁺ ions for one molecule of amine. Human VMAT2 is 514 amino acids (aa) in length. It contains two cytoplasmic domains, a 20 aa and a 52 aa N- and C-terminal respectively, plus an extended 88 aa luminal loop between aa 42-129. There is one luminal, intrachain disulfide bond that contributes to amine transport (C126-C333). In addition, residues in TM domains 5-8 (aa 220-352) and 9-12 (aa 358‑462) also contribute to high affinity ligand interaction. VMAT2 is constitutively phosphorylated by CKII on S511 and S513. Within the cytoplasmic C-terminus, human VMAT2 is 94% aa identical to rat VMAT2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.