VMAT2 Antibody (899327) [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: Flow
• Clone: 899327
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

VMAT2 Antibody (899327) [Alexa Fluor® 594] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human VMAT2
  Met1-Asp514
  Accession # Q05940
• Specificity: Detects human VMAT2 in ELISAs and Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC18A2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells
• Application Notes: Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
• Preservative: 0.09% Sodium Azide
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VMAT2 Antibody (899327) [Alexa Fluor® 594]

• MGC120477
• MNAT
• monoamine neurotransmitter transporter
• Monoamine transporter
• SLC18A2
• solute carrier family 18 (vesicular monoamine), member 2
• Solute carrier family 18 member 2
• SVAT
• SVMT
• SVMTMGC120478
• synaptic vesicular amine transporter
• VAT2
• VAT2MGC26538
• vesicle monoamine transporter type 2
• vesicle monoamine/H+ antiporter
• Vesicular amine transporter 2
• VMAT2

Background

The Vesicular Monoamine Transporter 2 (VMAT2), also known as VAT2 and SCL18A, is a 55-75 kDa member of the vesicular transporter family, a major facilitator superfamily. VMAT2 is a 12 transmembrane (TM) glycoprotein that is found in the membrane of brain neurosecretory vesicles. It transports monoamines (dopamine, serotonin, and particularly histamine) from the cytosol into secretion vesicles by exchanging two H⁺ ions for one molecule of amine. Human VMAT2 is 514 amino acids (aa) in length. It contains two cytoplasmic domains, a 20 aa and a 52 aa N- and C-terminal respectively, plus an extended 88 aa luminal loop between aa 42-129. There is one luminal, intrachain disulfide bond that contributes to amine transport (C126-C333). In addition, residues in TM domains 5-8 (aa 220-352) and 9-12 (aa 358‑462) also contribute to high affinity ligand interaction. VMAT2 is constitutively phosphorylated by CKII on S511 and S513. Within the cytoplasmic C-terminus, human VMAT2 is 94% aa identical to rat VMAT2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VMAT2 Antibody (FAB8327T)(1)

Publication using FAB8327T

• Stevan Pecic, Nenad Milosavic, Gina Rayat, Antonella Maffei, Paul E. Harris A novel optical tracer for VMAT2 applied to live cell measurements of vesicle maturation in cultured human beta -cells Scientific Reports 2019-04-01 [PMID: 30932004] (Immunohistochemistry, Human)

Bioinformatics

• Gene Symbol: SLC18A2
• Uniprot:
  • Q05940()