VGF Antibody (619707) [Unconjugated]

VGF was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Mouse Anti-Human/Mouse VGF Monoclonal Antibody (Catalog # MAB6317) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Product Discontinued

VGF Antibody (619707) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human VGF
  Thr554-Pro615
  Accession # O15240
• Specificity: Detects human and mouse VGF in direct ELISAs.
• Source: N/A
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: VGF
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1.7-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VGF Antibody (619707) [Unconjugated]

• neuro-endocrine specific protein VGF
• neurosecretory protein VGF
• VGF nerve growth factor inducible
• VGF

Background

Neurosecretory protein VGF (VGF) is a 65 kDa secreted protein that is expressed in the brain, where it may be involved in the regulation of cell-cell interactions or in synaptogenesis during maturation of the nervous system. Human VGF is synthesized as a 615 amino acid (aa) precursor that contains a 22 aa signal sequence and a 593 aa mature chain. The region including aa 353-447 is Asp/Glu-rich and thus an acidic region. Human VGF shares 87% aa sequence identity to mouse VGF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VGF
• Uniprot:
  • O15240()