![Western Blot: VEPH1 Antibody [NBP1-82291] - Analysis in control (vector only transfected HEK293T lysate) and VEPH1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize2/VEPH1-Antibody-Western-Blot-NBP1-82291-img0010.jpg "Western Blot: VEPH1 Antibody [NBP1-82291] - Analysis in control (vector only transfected HEK293T lysate) and VEPH1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: RAGDLFSLDDSEIEDSLTEALEQIKIISSSSDYQTNNNDQAVVEICITRITTAIRETESIEKHAKALVGLWDSCLEHNLRPFGKDEDTPHAKIASDIMSCILQNYNRPPVMALAIPIAVKFLHRGNKELCRNMS |
| Predicted Species | Mouse (93%), Rat (93%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | VEPH1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
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| Publication using NBP1-82291 | Applications | Species |
|---|---|---|
| Shi X, Xu C, Li Y et al. A novel role of VEPH1 in regulating AoSMC phenotypic switching J. Cell. Physiol. 2020-04-27 [PMID: 32342520] |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | VEPH1 |
![Immunocytochemistry/Immunofluorescence: VEPH1 Antibody [NBP1-82291] - Staining of human cell line A-431 shows localization to nucleus, nucleoli & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/VEPH1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82291-img0008.jpg "Immunocytochemistry/Immunofluorescence: VEPH1 Antibody [NBP1-82291] - Staining of human cell line A-431 shows localization to nucleus, nucleoli & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: VEPH1 Antibody [NBP1-82291] - Staining of human endometrium shows very weak membranous positivity in glandular cells.](http://images.novusbio.com/fullsize2/VEPH1-Antibody-Immunohistochemistry-Paraffin-NBP1-82291-img0015.jpg "Immunohistochemistry-Paraffin: VEPH1 Antibody [NBP1-82291] - Staining of human endometrium shows very weak membranous positivity in glandular cells.")
![Immunohistochemistry-Paraffin: VEPH1 Antibody [NBP1-82291] - Staining of human lung shows strong ctyoplasmic positivity in macrophages and alveolar cells.](http://images.novusbio.com/fullsize2/VEPH1-Antibody-Immunohistochemistry-Paraffin-NBP1-82291-img0011.jpg "Immunohistochemistry-Paraffin: VEPH1 Antibody [NBP1-82291] - Staining of human lung shows strong ctyoplasmic positivity in macrophages and alveolar cells.")
![Immunohistochemistry-Paraffin: VEPH1 Antibody [NBP1-82291] - Staining of human kidney shows strong membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize2/VEPH1-Antibody-Immunohistochemistry-Paraffin-NBP1-82291-img0012.jpg "Immunohistochemistry-Paraffin: VEPH1 Antibody [NBP1-82291] - Staining of human kidney shows strong membranous positivity in cells in tubules.")
![Immunohistochemistry-Paraffin: VEPH1 Antibody [NBP1-82291] - Staining of human adrenal gland shows strong membranous and cytoplasmic positivity in positivity in glandular cells.](http://images.novusbio.com/fullsize2/VEPH1-Antibody-Immunohistochemistry-Paraffin-NBP1-82291-img0013.jpg "Immunohistochemistry-Paraffin: VEPH1 Antibody [NBP1-82291] - Staining of human adrenal gland shows strong membranous and cytoplasmic positivity in positivity in glandular cells.")
![Immunohistochemistry-Paraffin: VEPH1 Antibody [NBP1-82291] - Staining of human fallopian tube shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize2/VEPH1-Antibody-Immunohistochemistry-Paraffin-NBP1-82291-img0014.jpg "Immunohistochemistry-Paraffin: VEPH1 Antibody [NBP1-82291] - Staining of human fallopian tube shows moderate membranous positivity in glandular cells.")
![Immunohistochemistry CFTR Antibody (24-1) [Unconjugated] - C-terminus](https://images.novusbio.com/images2/CFTR_MAB25031_Immunohistochemistry_6973.jpg)
![Immunohistochemistry CFTR Antibody (24-1) [Unconjugated] - C-terminus](https://images.novusbio.com/images2/CFTR_MAB25031_Immunohistochemistry_9671.jpg)
![t-Plasminogen Activator/tPA [HRP]](https://images.novusbio.com/images2/DATA_tPlasminogen_Activator_DTPA00_ELISA_854.jpg)
![t-Plasminogen Activator/tPA [HRP]](https://images.novusbio.com/images2/tPlasminogen_Activator_DTPA00_ELISA_510.jpg)
![Enzyme Activity u-Plasminogen Activator/Urokinase [Unconjugated]](https://images.novusbio.com/images2/uPlasminogen_Activator_1310SE_1837.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images2/Goat-anti-Rabbit-IgG-(H+L)-Secondary-Antibody-[HRP]-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images2/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")