VEGFR2/KDR/Flk-1 Antibody [Unconjugated]

VEGFR2/KDR/Flk-1 was detected in immersion fixed frozen sections of mouse embryo (14 d.p.c.) using 15 µg/mL Goat Anti-Mouse VEGFR2/KDR/Flk-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF644) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to mesenchymal cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

VEGFR2 was detected in acetone fixed cryosections of mouse kidney tissue using Goat Anti-Mouse VEGFR2/KDR/Flk-1 Polyclonal Antibody (Catalog # AF644) for 50 minutes at room temperature. Tissues were stained with rabbit anti-goat secondaryantibody and HRP polymer-conjugated anti-rabbit IgG followed by AEC+Substrate Chromogen (red) followed by counterstaining with hematoxylin (blue). Experiments were carried out and the image was provided by Dr. Grietje Molema, University Medical Center Groningen, The Netherlands.

Recombinant Mouse VEGFR2/KDR/Flk‑1 Fc Chimera (Catalog # 443-KD) inhibits Recombinant Mouse VEGF164 (Catalog # 493-MV) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse VEGF164 (5 ng/mL) activity elicited by Recombinant Mouse VEGFR2/KDR/Flk‑1 Fc Chimera (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse VEGFR2/KDR/Flk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF644). The ND50 is typically 0.1‑0.3 µg/mL.

BM-PC express Notch pathway ligands/receptors and show increased expression of notch downstream targets during endothelial differentiation.A. Expression of Notch receptors and ligands in BM-PC was detected by RT-PCR. B. Expression of Notch downstream targets (Hes 1, Hey 1 and 2) was detected at different time points during BM-PC endothelial differentiation by quantitative real-time PCR. C. Representative images (×200) of BM-PC at day 20 of culture showing positivity for endothelial lineage specific markers, acetylated LDL, CD31 , Flk-1 and vWF with DAPI nuclear counterstaining in blue. D. Quantification of BM-PC positive cells for acetylated LDL, CD31 , Flk-1 and VWF after 20 days of culture. Each experiment was performed in triplicate and the mean presented (n = 3). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0003752), licensed under a CC-BY license. Not internally tested by R&D Systems.

Vascular alterations after intraocular VEGF-A injection. a Morphology of IB4-stained P6 wild-type retinal vessels at 4 h after administration of human VEGF-A165 (0.5 µl at a concentration of 5 μg μl−1). Note blunt appearance of the vessel front after VEGF-A injection but not for vehicle (PBS) control. Scale bar, 200 µm. b Quantitation of sprouts and filopodia at the front of the P6 vessel plexus after injection of VEGF-A165 or vehicle control. Error bars, s.e.m. p-values, Student’s t-test. c PDGFR beta + (green) pericytes are unaffected by short-term VEGF-A administration, whereas VEGFR2 immunosignals (white) are increased in IB4+ (red) ECs (arrowheads). Images shown correspond to insets in a. Scale bar, 100 µm. d Quantitation of VEGFR2 immunosignals intensity in the peripheral plexus of P6 retinas after injection of VEGF-A165 or vehicle control. Error bars, s.e.m. p-values, Student’s t-test. e Confocal images showing increased Esm1 immunostaining (white) in IB4+ (red) ECs in the peripheral plexus (arrowheads) after VEGF-A injection in P6 pups. Scale bar, 200 µm. f VEGF-A165 injection-mediated increase of Esm1 immunosignals (normalized to IB4+ EC area) in the peripheral capillary plexus but not at the edge of the angiogenic front in comparison to PBS-injected controls at P6. Error bars, s.e.m. p-values, Student’s t-test. NS, not statistically significant. g Short-term VEGF-A165 administration leads to clustering of Erg1+ (green) and IB4+ (red) ECs, as indicated, in thick sprout-like structures of P6 retinas. Panels in the center and on the right (scale bar, 20 µm) show higher magnification of the insets on the left (scale bar, 100 µm). Dashed lines in panels on the right outline IB4+ vessels. h Quantitation of EC density in the leading front vessel and emerging sprouts of the P6 angiogenic front after injection of VEGF-A165 or vehicle control. Error bars, s.e.m. p-values, Student’s t-test Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29146905), licensed under a CC-BY license. Not internally tested by R&D Systems.

Inactivation of Flt1 in PDGFR beta + cells. a Experimental scheme of tamoxifen administration for the generation of Flt1iPC mutants. b P6 control, Flt1iPC/+ and Flt1iPC retinas stained with isolectin B4 (IB4). Dashed circles indicate vessel-covered (yellow) and peripheral avascular (white) areas in the overview pictures (top). Scale bar, 500 µm. c Quantitation of body weight and radial outgrowth of the retinal vasculature in control, Flt1iPC/+ and Flt1iPC P6 pups. Error bars, s.e.m. p-values, one-way ANOVA. NS, not statistically significant. d Confocal images of the IB4-stained P6 control, Flt1iPC/+ and Flt1iPC retinal angiogenic front illustrating differences in sprout number and morphology. Scale bar, 100 µm. e Quantitation of sprouts and filopodia in P6 control, Flt1iPC/+ and Flt1iPC retinas. Error bars, s.e.m. p-values, one-way ANOVA and Tukey’s multiple comparison test. NS, not statistically significant. f Confocal images of IB4 (red), Erg1 (green) and VEGFR2 (white) stained P6 retinas highlighting the accumulation of EC nuclei and enhanced VEGFR2 immunosignals (arrowheads) in Flt1iPC sprouts. Vessels are outlined by dashed lines on the right panel. Scale bar, 100 µm. g Quantitation of EC proliferation (EdU+ Erg1+) at the angiogenic front, EC density in sprouts and leading front vessel and VEGFR2 immunosignals intensity in the angiogenic front of control and Flt1iPC P6 retinas. Error bars, s.e.m. p-values, Student’s t-test. h Esm1 (white) expression (arrowheads) in the angiogenic front (IB4+, red, first two columns) and detection of desmin+ pericytes (green, third column) in P6 control and Flt1iPC retinas. Scale bar, 100 µm. i Quantitation of Esm1+ proportion relative to vascular area (IB4+) in the angiogenic front of control and Flt1iPC P6 retinas. Error bars, s.e.m. p-values, Student’s t-test. j Confocal images of P6 retinas stained for NG2 (green) and IB4 (red) showing no significant changes in pericyte coverage in the front (first two columns) or the remodeling plexus around veins (v) or arteries (a) (last two columns). Scale bar, 100 µm. k, l Quantitation of pericyte coverage k and relative gene expression by qPCR on whole lysates l in control and Flt1iPC P6 retinas. Error bars, s.e.m. p-values, Student’s t-test. NS, not statistically significant Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29146905), licensed under a CC-BY license. Not internally tested by R&D Systems.

Formalin-fixed paraffin-embedded tissue sections of mouse kidney were probed for VEGFR2 mRNA (ACD RNAScope Probe, catalog #414818, Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using goat anti-mouse VEGFR2 polyclonal antibody (R&D Systems catalog # AF644) at 1.7ug/mL with 1 hour incubation at room temperature followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to glomeruli and fibroblasts.

BM-PC express Notch pathway ligands/receptors and show increased expression of notch downstream targets during endothelial differentiation.A. Expression of Notch receptors and ligands in BM-PC was detected by RT-PCR. B. Expression of Notch downstream targets (Hes 1, Hey 1 and 2) was detected at different time points during BM-PC endothelial differentiation by quantitative real-time PCR. C. Representative images (×200) of BM-PC at day 20 of culture showing positivity for endothelial lineage specific markers, acetylated LDL, CD31 , Flk-1 and vWF with DAPI nuclear counterstaining in blue. D. Quantification of BM-PC positive cells for acetylated LDL, CD31 , Flk-1 and VWF after 20 days of culture. Each experiment was performed in triplicate and the mean presented (n = 3). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/19015735), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB, Flow, IHC, B/N, CyTOF-ready, Dual ISH-IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

VEGFR2/KDR/Flk-1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse VEGFR2
  Ala20-Glu762
  Accession # P35918
• Specificity: Detects mouse VEGFR2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human VEGFR2 is observed and less than 2% cross-reactivity with recombinant mouse (rm) VEGFR1 and rmVEGFR3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KDR
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Dual RNAscope ISH-IHC 5-15 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.1-0.3 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGFR2/KDR/Flk-1 Antibody [Unconjugated]

• CD309 antigen
• CD309
• EC 2.7.10
• EC 2.7.10.1
• Fetal liver kinase 1
• fetal liver kinase-1
• Flk1
• Flk-1
• FLK1tyrosine kinase growth factor receptor
• KDR
• kinase insert domain receptor (a type III receptor tyrosine kinase)
• Kinase insert domain receptor
• KRD1
• Ly73
• Protein-tyrosine kinase receptor flk-1
• soluble VEGFR2
• vascular endothelial growth factor receptor 2
• VEGF R2
• VEGFR
• VEGFR2
• VEGFR-2

Background

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1), and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.

Mouse VEGFR2 cDNA encodes a 1367 amino acid (aa) residue precursor protein with a 19 aa residue signal peptide. Mature VEGFR2 is composed of a 743 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 583 aa residue cytoplasmic domain. In contrast to VEGFR1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGFR2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.
2. Achen, M.G. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VEGFR2/KDR/Flk-1 Antibody (AF644)(101)

Publications using AF644

• Wang, H;Barry, K;Zaini, A;Coakley, G;Moyat, M;Daunt, CP;Wickramasinghe, LC;Azzoni, R;Chatzis, R;Yumnam, B;Camberis, M;Le Gros, G;Perdijk, O;Foong, JPP;Bornstein, JC;Marsland, BJ;Harris, NL; Helminth infection driven gastrointestinal hypermotility is independent of eosinophils and mediated by alterations in smooth muscle instead of enteric neurons PLoS pathogens 2024-08-01 [PMID: 39141685] (Immunohistochemistry, Mouse, Transgenic Mouse)
• Jannaway, M;Iyer, D;Mastrogiacomo, DM;Li, K;Sung, DC;Yang, Y;Kahn, ML;Scallan, JP; VEGFR3 is required for button junction formation in lymphatic vessels Cell reports 2023-07-15 [PMID: 37454290] (IHC, Mouse)
• Gauri Arolkar, Sneha K. Krishna Kumar, Hanjay Wang, Karen M. Gonzalez, Suraj Kumar, Bhavnesh Bishnoi et al. Dedifferentiation and Proliferation of Artery Endothelial Cells Drive Coronary Collateral Development in Mice Arteriosclerosis, Thrombosis, and Vascular Biology 8/1/2023 [PMID: 37345524]
• Wang J, Rattner A, Nathans J Bacterial meningitis in the early postnatal mouse studied at single-cell resolution eLife 6/15/2023 [PMID: 37318981]
• F Mueller-Di, W Lederle, A Rix, S Koletnik, D Doleschel, M Snelting, F Gremse, F Kiessling Molecular Ultrasound Imaging Depicts the Modulation of Tumor Angiogenesis by Acetylsalicylic Acid International Journal of Molecular Sciences, 2023-04-11;24(8):. 2023-04-11 [PMID: 37108226] (IHC, Mouse)
• Chenguang Wang, Yalan Wu, Yangxian Li, Yang Zhang, Yee Lok Fung, Ka Kui Tong et al. Smad4-mediated angiogenesis facilitates the beiging of white adipose tissue in mice iScience 3/17/2023 [PMID: 36915676]
• E Lee, EA Lee, E Kong, H Chon, M Llaiqui-Co, CH Park, BY Park, NR Kang, JS Yoo, HS Lee, HS Kim, SH Park, SW Choi, D Vestweber, JH Lee, P Kim, WS Lee, I Kim An agonistic anti-Tie2 antibody suppresses the normal-to-tumor vascular transition in the glioblastoma invasion zone Experimental & Molecular Medicine, 2023-02-24;55(2):470-484. 2023-02-24 [PMID: 36828931] (IHC, Mouse)
• Chi-Hsiu Liu, Felix Yemanyi, Kiran Bora, Neetu Kushwah, Alexandra K. Blomfield, Theodore M. Kamenecka et al. Genetic deficiency and pharmacological modulation of ROR alpha regulate laser-induced choroidal neovascularization Aging (Albany NY) 1/15/2023 [PMID: 36626253]
• Jeff Hsu, Colin Chong, Jeffrey Serrill, Levina Goon, Joan Balayan, Eric N. Johnson et al. Preclinical Characterization of XL092, a Novel Receptor Tyrosine Kinase Inhibitor of MET, VEGFR2, AXL, and MER Molecular Cancer Therapeutics 2/1/2023 [PMID: 36399631]
• Motaharehsadat Heydarian, Prajakta Oak, Xin Zhang, Nona Kamgari, Alida Kindt, Markus Koschlig et al. Relationship between impaired BMP signalling and clinical risk factors at early-stage vascular injury in the preterm infant Thorax 12/1/2022 [PMID: 35580897]
• Chengqian Yang, Xingbo Feng, Zengxian Li, Qingsi He Plumbagin inhibits tumor angiogenesis of gastric carcinoma in mice by modulating nuclear factor-kappa B pathway Translational Cancer Research 2/1/2020 [PMID: 35117400]
• Peter Baluk, Donald M. McDonald Imaging Blood Vessels and Lymphatics in Mouse Trachea Wholemounts Methods in Molecular Biology 9/6/2022 [PMID: 35099733]
• Sinem Karaman, Satu Paavonsalo, Krista Heinolainen, Madeleine H. Lackman, Amanda Ranta, Karthik A. Hemanthakumar et al. Interplay of vascular endothelial growth factor receptors in organ-specific vessel maintenance Journal of Experimental Medicine 3/7/2022 [PMID: 35050301]
• JM Arpino, H Yin, EK Prescott, SCR Staples, Z Nong, F Li, J Chevalier, B Balint, C O'Neil, R Mortuza, S Milkovich, JJ Lee, D Lorusso, M Sandig, DW Hamilton, DW Holdsworth, TL Poepping, CG Ellis, JG Pickering Low-flow intussusception and metastable VEGFR2 signaling launch angiogenesis in ischemic muscle Science Advances, 2021-11-26;7(48):eabg9509. 2021-11-26 [PMID: 34826235] (IHC, Mouse)
• F Progatzky, M Shapiro, SH Chng, B Garcia-Cas, CH Classon, S Sevgi, A Laddach, AC Bon-Frauch, R Lasrado, M Rahim, EM Amaniti, S Boeing, K Shah, LJ Entwistle, A Suárez-Bon, MS Wilson, B Stockinger, V Pachnis Regulation of intestinal immunity and tissue repair by enteric glia Nature, 2021-10-20;599(7883):125-130. 2021-10-20 [PMID: 34671159] (IHC, Mouse)
• Evripidis Lanitis, Paris Kosti, Catherine Ronet, Elisabetta Cribioli, Giorgia Rota, Aodrenn Spill et al. VEGFR-2 redirected CAR-T cells are functionally impaired by soluble VEGF-A competition for receptor binding Journal for ImmunoTherapy of Cancer 8/13/2021 [PMID: 34389616]
• Sophie M Morgani, Jie Su, Jennifer Nichols, Joan Massagué, Anna-Katerina Hadjantonakis The transcription factor Rreb1 regulates epithelial architecture, invasiveness, and vasculogenesis in early mouse embryos eLife 4/30/2021 [PMID: 33929320]
• DH Yeom, YS Lee, I Ryu, S Lee, B Sung, HB Lee, D Kim, JH Ahn, E Ha, YS Choi, SH Lee, WK You ABL001, a Bispecific Antibody Targeting VEGF and DLL4, with Chemotherapy, Synergistically Inhibits Tumor Progression in Xenograft Models International Journal of Molecular Sciences, 2020-12-29;22(1):. 2020-12-29 [PMID: 33383646] (IHC, Mouse)
• Anja Nitzsche, Riikka Pietilä, Dominic T Love, Chiara Testini, Takeshi Ninchoji, Ross O Smith et al. Paladin is a phosphoinositide phosphatase regulating endosomal VEGFR2 signalling and angiogenesis EMBO reports 2021-02-03 [PMID: 33369848] (Immunoprecipitation, Neutralization, Immunocytochemistry, Human, Mouse)
• Anja Nitzsche, Riikka Pietilä, Dominic T Love, Chiara Testini, Takeshi Ninchoji, Ross O Smith et al. Paladin is a phosphoinositide phosphatase regulating endosomal VEGFR2 signalling and angiogenesis EMBO reports 2/3/2021 [PMID: 33369848]
• Grzegorz Gula, Sławomir Rumiński, Justyna Niderla-Bielińska, Agnieszka Jasińska, Ewelina Kiernozek, Ewa Jankowska-Steifer et al. Potential functions of embryonic cardiac macrophages in angiogenesis, lymphangiogenesis and extracellular matrix remodeling Histochemistry and Cell Biology 1/1/2021 [PMID: 33130914]
• SP Hong, MJ Yang, H Cho, I Park, H Bae, K Choe, SH Suh, RH Adams, K Alitalo, D Lim, GY Koh Distinct fibroblast subsets regulate lacteal integrity through YAP/TAZ-induced VEGF-C in intestinal villi Nat Commun, 2020-08-14;11(1):4102. 2020-08-14 [PMID: 32796823] (IHC, Mouse)
• L Su, X Lei, H Ma, C Feng, J Jiang, J Jiao PRDM16 orchestrates angiogenesis via neural differentiation in the developing brain Cell Death Differ., 2020-02-03;0(0):. 2020-02-03 [PMID: 32015502] (IHC, Mouse)
• E Harde, L Nicholson, B Furones Cu, D Bissen, S Wigge, S Urban, M Segarra, C Ruiz de Al, A Acker-Palm EphrinB2 regulates VEGFR2 during dendritogenesis and hippocampal circuitry development Elife, 2019-12-23;8(0):. 2019-12-23 [PMID: 31868584] (IHC, Mouse)
• Robert Luck, Severino Urban, Andromachi Karakatsani, Eva Harde, Sivakumar Sambandan, LaShae Nicholson et al. VEGF/VEGFR2 signaling regulates hippocampal axon branching during development eLife 12/23/2019 [PMID: 31868583]
• D Duc, S Vigne, J Bernier-La, Y Yersin, F Ruiz, N Gaïa, S Leo, V Lazarevic, J Schrenzel, TV Petrova, C Pot Disrupting Myelin-Specific Th17 Cell Gut Homing Confers Protection in an Adoptive Transfer Experimental Autoimmune Encephalomyelitis Cell Rep, 2019-10-08;29(2):378-390.e4. 2019-10-08 [PMID: 31597098] (IHC, Mouse)
• Morales-Navarrete H, Nonaka H et al. Liquid-crystal organization of liver tissue. Elife 6/17/2019 [PMID: 31204997]
• S Hendrikx, S Coso, B Prat-Luri, L Wetterwald, A Sabine, CA Franco, S Nassiri, N Zangger, H Gerhardt, M Delorenzi, TV Petrova Endothelial Calcineurin Signaling Restrains Metastatic Outgrowth by Regulating Bmp2 Cell Rep, 2019-01-29;26(5):1227-1241.e6. 2019-01-29 [PMID: 30699351] (IHC, Mouse)
• Soumyashree Das, Andrew B. Goldstone, Hanjay Wang, Justin Farry, Gaetano D’Amato, Michael J. Paulsen et al. A Unique Collateral Artery Development Program Promotes Neonatal Heart Regeneration Cell 2/1/2019 [PMID: 30686582]
• Gregory Farber, Matthew M. Parks, Nicole Lustgarten Guahmich, Yi Zhang, Sébastien Monette, Scott C. Blanchard et al. ADAM10 controls the differentiation of the coronary arterial endothelium Angiogenesis 2019-05-01 [PMID: 30446855] (Immunohistochemistry, Transgenic Mouse)
• Gregory Farber, Matthew M. Parks, Nicole Lustgarten Guahmich, Yi Zhang, Sébastien Monette, Scott C. Blanchard et al. ADAM10 controls the differentiation of the coronary arterial endothelium Angiogenesis 5/1/2019 [PMID: 30446855]
• Alexis M Ceasrine, Eugene E Lin, David N Lumelsky, Radhika Iyer, Rejji Kuruvilla Adrb2 controls glucose homeostasis by developmental regulation of pancreatic islet vasculature eLife 10/10/2018 [PMID: 30303066]
• Alice Plein, Alessandro Fantin, Laura Denti, Jeffrey W. Pollard, Christiana Ruhrberg Erythro-myeloid progenitors contribute endothelial cells to blood vessels Nature 2018-10-11 [PMID: 30258231] (Western Blot, Mouse)
• Alice Plein, Alessandro Fantin, Laura Denti, Jeffrey W. Pollard, Christiana Ruhrberg Erythro-myeloid progenitors contribute endothelial cells to blood vessels Nature 10/11/2018 [PMID: 30258231]
• Peter Baluk, Donald M. McDonald Imaging Lymphatics in Mouse Lungs Methods in Molecular Biology 4/29/2019 [PMID: 30242759]
• C Choquet, THM Nguyen, P Sicard, E Buttigieg, TT Tran, F Kober, I Varlet, R Sturny, MW Costa, RP Harvey, C Nguyen, P Rihet, S Richard, M Bernard, RG Kelly, N Lalevée, L Miquerol Deletion of Nkx2-5 in trabecular myocardium reveals the developmental origins of pathological heterogeneity associated with ventricular non-compaction cardiomyopathy PLoS Genet., 2018-07-06;14(7):e1007502. 2018-07-06 [PMID: 29979676] (IHC, Mouse)
• JM Rosin, DM Kurrasch In utero electroporation induces cell death and alters embryonic microglia morphology and expression signatures in the developing hypothalamus J Neuroinflammation, 2018-06-12;15(1):181. 2018-06-12 [PMID: 29895301] (IHC-Fr, Mouse)
• Y Zhang, MH Ulvmar, L Stanczuk, I Martinez-C, M Frye, K Alitalo, T Mäkinen Heterogeneity in VEGFR3 levels drives lymphatic vessel hyperplasia through cell-autonomous and non-cell-autonomous mechanisms Nat Commun, 2018-04-03;9(1):1296. 4/3/2018 [PMID: 29615616]
• IK Kim, K Kim, E Lee, DS Oh, CS Park, S Park, JM Yang, JH Kim, HS Kim, DT Shima, JH Kim, SH Hong, YH Cho, YH Kim, JB Park, GY Koh, YS Ju, HK Lee, S Lee, I Kim Sox7 promotes high-grade glioma by increasing VEGFR2-mediated vascular abnormality J. Exp. Med., 2018-02-14;0(0):. 2018-02-14 [PMID: 29444818] (IHC-P, Mouse)
• S Rhee, JI Chung, DA King, G D'amato, DT Paik, A Duan, A Chang, D Nagelberg, B Sharma, Y Jeong, M Diehn, JC Wu, AJ Morrison, K Red-Horse Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease Nat Commun, 2018-01-25;9(1):368. 2018-01-25 [PMID: 29371594] (IHC, Mouse)
• CJ Drummond, JA Hanna, MR Garcia, DJ Devine, AJ Heyrana, D Finkelstei, JE Rehg, ME Hatley Hedgehog Pathway Drives Fusion-Negative Rhabdomyosarcoma Initiated From Non-myogenic Endothelial Progenitors Cancer Cell, 2018-01-08;33(1):108-124.e5. 2018-01-08 [PMID: 29316425] (IHC, Mouse)
• JL Sones, AA Merriam, A Seffens, DA Brown-Gran, SD Butler, AM Zhao, X Xu, CJ Shawber, JK Grenier, NC Douglas Angiogenic factor imbalance precedes complement deposition in placentae of the BPH/5 model of preeclampsia FASEB J., 2018-01-08;0(0):. 2018-01-08 [PMID: 29279353] (IHC, Mouse)
• Morgane Sonia Thion, Donovan Low, Aymeric Silvin, Jinmiao Chen, Pauline Grisel, Jonas Schulte-Schrepping et al. Microbiome Influences Prenatal and Adult Microglia in a Sex-Specific Manner Cell 1/25/2018 [PMID: 29275859]
• Y Chen, H Xu, G Lin Generation of iPSC-derived limb progenitor-like cells for stimulating phalange regeneration in the adult mouse Cell Discov, 2017-12-19;3(0):17046. 2017-12-19 [PMID: 29263795] (IHC, Mouse)
• J Kim, DY Park, H Bae, DY Park, D Kim, CK Lee, S Song, TY Chung, DH Lim, Y Kubota, YK Hong, Y He, HG Augustin, G Oliver, GY Koh Impaired angiopoietin/Tie2 signaling compromises Schlemm&#039;s canal integrity and induces glaucoma J. Clin. Invest., 2017-09-18;0(0):. 2017-09-18 [PMID: 28920924] (IHC, Mouse)
• Dina Vara, Joanna M. Watt, Tiago M. Fortunato, Harry Mellor, Matthew Burgess, Kate Wicks et al. Direct Activation of NADPH Oxidase 2 by 2-Deoxyribose-1-Phosphate Triggers Nuclear Factor Kappa B-Dependent Angiogenesis Antioxidants & Redox Signaling 1/10/2018 [PMID: 28793782]
• Andrew H. Chang, Brian C. Raftrey, Gaetano D'Amato, Vinay N. Surya, Aruna Poduri, Heidi I. Chen et al. DACH1 stimulates shear stress-guided endothelial cell migration and coronary artery growth through the CXCL12–CXCR4 signaling axis Genes & Development 7/1/2017 [PMID: 28779009]
• H Wu, J Zhao, B Fu, S Yin, C Song, J Zhang, S Zhao, Y Zhang Retinoic acid-induced upregulation of miR-219 promotes the differentiation of embryonic stem cells into neural cells Cell Death Dis, 2017-07-27;8(7):e2953. 2017-07-27 [PMID: 28749472] (Western Blot, Mouse)
• K Heinolaine, S Karaman, G D'Amico, T Tammela, R Sormunen, L Eklund, K Alitalo, G Zarkada VEGFR3 Modulates Vascular Permeability by Controlling VEGF/VEGFR2 Signaling Circ. Res, 2017-03-15;0(0):. 2017-03-15 [PMID: 28298294] (Western Blot, Mouse)
• UH Langen, ME Pitulescu, JM Kim, R Enriquez-G, KK Sivaraj, AP Kusumbe, A Singh, J Di Russo, MG Bixel, B Zhou, L Sorokin, JM Vaquerizas, RH Adams Cell-matrix signals specify bone endothelial cells during developmental osteogenesis Nat. Cell Biol, 2017-02-20;19(3):189-201. 2017-02-20 [PMID: 28218908] (IHC, Mouse)
• Tsuruhito Tanaka, Nirmal Panthee, Yoshifumi Itoda, Naoko Yamauchi, Masashi Fukayama, Minoru Ono Negative pressure wound therapy induces early wound healing by increased and accelerated expression of vascular endothelial growth factor receptors European Journal of Plastic Surgery 8/1/2016 [PMID: 27512293]
• MH Ulvmar, I Martinez-C, L Stanczuk, T Mäkinen Pdgfrb-Cre targets lymphatic endothelial cells of both venous and non-venous origins Genesis, 2016-04-21;0(0):. 2016-04-21 [PMID: 27060598] (IHC, Mouse)
• Katharina S Volz, Andrew H Jacobs, Heidi I Chen, Aruna Poduri, Andrew S McKay, Daniel P Riordan et al. Pericytes are progenitors for coronary artery smooth muscle eLife 10/19/2015 [PMID: 26479710]
• Ingrid Moen, Matthew Gebre, Vanesa Alonso-Camino, Debbie Chen, David Epstein, Donald M. McDonald Anti-metastatic action of FAK inhibitor OXA-11 in combination with VEGFR-2 signaling blockade in pancreatic neuroendocrine tumors Clinical & Experimental Metastasis 12/1/2015 [PMID: 26445848]
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Reviews for VEGFR2/KDR/Flk-1 Antibody (AF644) (3)

Average Rating: 4.3

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Mouse.

reviewed by: Bhavin Shah Immunofluorescence -Frozen tissue sections Mouse 04/28/2020

Summary

• Application: Other
• Sample Tested: Brain,Embryonic brain
• Species: Mouse
• Lot: COA0412081

Comments

• Comments: The cryo frozen samples were blocked with 10% NDS + 0.5% triton-x100 in PBS for 2 hours before using Primary antibody ( in the same blocking buffer) at 4 degrees overnight. Secondary AF488 was used for 2h at RT

reviewed by: Verified Customer IHC-Fr Mouse 01/09/2015

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: See PMID 23746980
• Species: Mouse

reviewed by: Verified Customer IF Mouse 01/09/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 23746980
• Species: Mouse

Bioinformatics

• Gene Symbol: KDR
• Uniprot:
  • P35918()