VEGFR1/Flt-1 Antibody (141522) [Phycoerythrin]

bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse VEGF R1/Flt-1 PE-conjugated Monoclonal Antibody (Catalog # FAB4711P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Fibroblast-derived VEGF enriches VEGFR1+ cells bone marrow and pre-metastatic sites.(a) Immunohistochemical expression of mouse VEGF in the tumour xenografts established with KYSE150-Id1, KYSE150-Id1-shIGF2 or vector control cells (scale bar, 20 μm). (b) Flow cytometry data showing the expression of VEGFR1+ cells in bone marrow (upper panel) and lungs (lower panel) of mice with subcutaneous implantation of indicated fibroblasts and treatment (female 6–8-week-old nude mice, n=3 per group). (c,d) Flow cytometry analysis of VEGFR1+ cells (c) and comparison of tumour volume (d) of subcutaneous tumour xenografts established from co-implantation of KYSE150 cells and indicated NEFs in the presence or absence of Avastin treatment. Photographs show representative tumours of the three groups (female 6–8-week-old nude mice, n=6 per group; scale bar, 1 cm). Bars, s.d.; *P<0.05; **P<0.01; ***P<0.001 by Student's t-test. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms14399), licensed under a CC-BY license. Not internally tested by R&D Systems.

Fibroblast-derived VEGF enriches VEGFR1+ cells bone marrow and pre-metastatic sites.(a) Immunohistochemical expression of mouse VEGF in the tumour xenografts established with KYSE150-Id1, KYSE150-Id1-shIGF2 or vector control cells (scale bar, 20 μm). (b) Flow cytometry data showing the expression of VEGFR1+ cells in bone marrow (upper panel) and lungs (lower panel) of mice with subcutaneous implantation of indicated fibroblasts and treatment (female 6–8-week-old nude mice, n=3 per group). (c,d) Flow cytometry analysis of VEGFR1+ cells (c) and comparison of tumour volume (d) of subcutaneous tumour xenografts established from co-implantation of KYSE150 cells and indicated NEFs in the presence or absence of Avastin treatment. Photographs show representative tumours of the three groups (female 6–8-week-old nude mice, n=6 per group; scale bar, 1 cm). Bars, s.d.; *P<0.05; **P<0.01; ***P<0.001 by Student's t-test. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms14399), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: Flow
• Clone: 141522
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Phycoerythrin

VEGFR1/Flt-1 Antibody (141522) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse VEGF R1/Flt‑1
  Ser27-Glu759
  Accession # P35969
• Specificity: Detects mouse VEGF R1/Flt-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human VEGF R1, recombinant mouse (rm) VEGF R2, or rmVEGF R3 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: FLT1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  12 months from date of receipt, 2 to 8 degreesC as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGFR1/Flt-1 Antibody (141522) [Phycoerythrin]

• EC 2.7.10
• EC 2.7.10.1
• FLT
• FLT1
• Flt-1
• Fms-like tyrosine kinase 1
• fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor)
• FRT
• Tyrosine-protein kinase FRT
• Tyrosine-protein kinase receptor FLT
• vascular endothelial growth factor receptor 1
• Vascular permeability factor receptor
• VEGF R1
• VEGFR1
• VEGFR-1

Background

VEGF R1 is one of the five receptor tyrosine kinases (RTKs) (VEGF R1, KDR/Flk-1, Flt-4, Tie-1, and Tek/Tie-2) whose expression is almost exclusively restricted to the endothelial cells. Tie-1 and tek/tie-2 define a new class of RTKs containing two immunoglobulin-like domains, three EGF homology domains and three fibronectin type III domains in their extracellular regions. VEGF R1/Flt-1, VEGF R2/KDR/Flk-1, VEGF R3/Flt-4 are members of the class III subfamily of RTKs containing seven immunoglobulin-like repeats in their extracellular domains. All five RTKs are likely to play central roles in vasculogenesis and angiogenesis. Full length mouse VEGF R1 mRNA encodes a 1333 amino acid (aa) residue precursor with a predicted 22 aa residue signal peptide. Mature VEGF R1 is composed of a 737 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 552 aa residue cytoplasmic domain. As a result of alternative splicing of the mRNA, a cDNA encoding a truncated form of VEGF R1, lacking the seventh immunoglobulin-like domain, the transmembrane and intracellular domains, has been cloned. The recombinant soluble VEGF R1/Fc chimera binds VEGF and PlGF with high affinity and is a potent VEGF antagonist.

1. He, Y. et al. (1999) Molecular Endocrinology 13:537.
2. Ferrara, N. and T. Davis-Smyth (1997) Endocrine Reviews 8:4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VEGFR1/Flt-1 Antibody (FAB4711P)(9)

Publications using FAB4711P

• X Liu, Z Li, J Sun, Z Zhang, W Li Interaction between PD-L1 and soluble VEGFR1 in glioblastoma-educated macrophages BMC Cancer, 2023-03-20;23(1):259. 2023-03-20 [PMID: 36941554] (Flow Cytometry, Mouse)
• Theivanthiran B, Yarla N, Haykal T et al. Tumor-intrinsic NLRP3-HSP70-TLR4 axis drives premetastatic niche development and hyperprogression during anti-PD-1 immunotherapy Science Translational Medicine 2022-11-23 [PMID: 36417489]
• D Yamakawa, W Jia, H Kidoya, S Hosojima, M Torigata, L Zhang, N Takakura Visualization of Proliferative Vascular Endothelial Cells in Tumors in�Vivo by Imaging Their Partner of Sld5-1 Promoter Activity Am. J. Pathol., 2018-04-09;0(0):. 2018-04-09 [PMID: 29650228] (Flow Cytometry, Mouse)
• WW Xu, B Li, XY Guan, SK Chung, Y Wang, YL Yip, SY Law, KT Chan, NP Lee, KW Chan, LY Xu, EM Li, SW Tsao, QY He, AL Cheung Cancer cell-secreted IGF2 instigates fibroblasts and bone marrow-derived vascular progenitor cells to promote cancer progression Nat Commun, 2017-02-10;8(0):14399. 2017-02-10 [PMID: 28186102] (Flow Cytometry, Mouse)
• Wen Wen Xu, Bin Li, Alfred K Y Lam, Sai Wah Tsao, Simon Y K Law, Kwok Wah Chan et al. Targeting VEGFR1- and VEGFR2-expressing non-tumor cells is essential for esophageal cancer therapy Oncotarget 2015-01-30 [PMID: 25595897] (Flow Cytometry, Mouse, Xenograft)
• Ohkubo , Hirotoki, Ito , Yoshiya, Minamino , Tsutomu, Eshima , Koji, Kojo , Ken, Okizaki , Shin-ich, Hirata , Mitsuhir, Shibuya , Masabumi, Watanabe , Masahiko, Majima , Masataka VEGFR1-positive macrophages facilitate liver repair and sinusoidal reconstruction after hepatic ischemia/reperfusion injury. PLoS ONE, 2014-08-27;9(8):e105533. 2014-08-27 [PMID: 25162491] (Flow Cytometry, Mouse)
• Sprague L, Muccioli M, Pate M, Singh M, Xiong C, Ostermann A, Niese B, Li Y, Li Y, Courreges M, Benencia F Dendritic cells: In vitro culture in two- and three-dimensional collagen systems and expression of collagen receptors in tumors and atherosclerotic microenvironments. Exp Cell Res, 2014-02-22;323(1):7-27. 2014-02-22 [PMID: 24569142] (Flow Cytometry, Mouse)
• Imoukhuede , Princess, Popel , Aleksand Expression of VEGF receptors on endothelial cells in mouse skeletal muscle. PLoS ONE, 2012-09-12;7(9):e44791. 2012-09-12 [PMID: 22984559] (Flow Cytometry, Mouse)
• Reddy K, Zhou Z, Schadler K Bone marrow subsets differentiate into endothelial cells and pericytes contributing to Ewing&#039;s tumor vessels. Mol. Cancer Res., 2008-06-01;6(6):929-36. 2008-06-01 [PMID: 18567797] (Flow Cytometry, Mouse)

Reviews for VEGFR1/Flt-1 Antibody (FAB4711P) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Mouse, Rat.

reviewed by: Verified Customer Flow Rat 01/26/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID: 21447646
• Species: Rat

reviewed by: Verified Customer Flow Mouse 01/26/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID: 24449499
• Species: Mouse

Bioinformatics

• Gene Symbol: FLT1
• Uniprot:
  • P35969()