VEGFR1/Flt-1 Antibody (141515) [Unconjugated]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clone
141515
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

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VEGFR1/Flt-1 Antibody (141515) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse VEGFR1
Ser27-Glu759
Accession # P35969
Specificity
Detects mouse VEGFR1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human VEGFR1, recombinant mouse (rm) VEGFR2, or rmVEGFR3 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
FLT1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB471 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGFR1/Flt-1 Antibody (141515) [Unconjugated]

  • EC 2.7.10
  • EC 2.7.10.1
  • FLT
  • FLT1
  • Flt-1
  • Fms-like tyrosine kinase 1
  • fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor)
  • FRT
  • Tyrosine-protein kinase FRT
  • Tyrosine-protein kinase receptor FLT
  • vascular endothelial growth factor receptor 1
  • Vascular permeability factor receptor
  • VEGF R1
  • VEGFR1
  • VEGFR-1

Background

VEGFR1 (also called Flt-1) is one of the five receptor tyrosine kinases (RTKs) (VEGFR1, KDR/Flk-1, Flt-4, Tie-1, and Tek/Tie-2) whose expression is almost exclusively restricted to the endothelial cells. Tie-1 and Tie-2/Tek define a class of RTKs containing two immunoglobulin-like domains, three EGF homology domains and three fibronectin type III domains in their extracellular regions. VEGFR1/Flt-1, VEGFR2/KDR/Flk-1, VEGFR3/Flt-4 are members of the class III subfamily of RTKs containing seven immunoglobulin-like repeats in their extracellular domains. All five RTKs are likely to play central roles in vasculogenesis and angiogenesis.

Full length mouse VEGFR1 mRNA encodes a 1333 amino acid (aa) residue precursor with a 22 aa residue signal peptide. Mature VEGFR1 is composed of a 737 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 552 aa residue cytoplasmic domain. As a result of alternative splicing of the mRNA, a cDNA encoding a truncated form of VEGFR1, lacking the seventh immunoglobulin-like domain, the transmembrane and intracellular domains, has been cloned. The recombinant soluble VEGFR1/Fc chimera binds VEGF and PlGF with high affinity and is a potent VEGF antagonist.

  1. He, Y. et al. (1999) Molecular Endocrinology 13:537.
  2. Ferrara, N. and T. Davis-Smyth (1997) Endocrine Reviews 8:4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VEGFR1/Flt-1 Antibody (MAB471)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Flow Cytometry, IHC, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
Flow Cytometry
(2)
IHC
(1)
IHC-P
(1)
Immunoprecipitation
(1)
Western Blot
(3)
All Applications
Filter By Species
Human
(2)
Mouse
(6)
All Species
Showing Publications 1 - 8 of 8.
Publications using MAB471 Applications Species
J Sarkar, Y Luo, Q Zhou, E Ivakhnitsk, D Lara, E Katz, MG Sun, V Guaiquil, M Rosenblatt VEGF receptor heterodimers and homodimers are differentially expressed in neuronal and endothelial cell types PLoS ONE, 2022;17(7):e0269818. 2022 [PMID: 35862373] (Immunoprecipitation, Human) Immunoprecipitation Human
AX Xie, N Iguchi, TC Clarkson, AP Malykhina Pharmacogenetic inhibition of lumbosacral sensory neurons alleviates visceral hypersensitivity in a mouse model of chronic pelvic pain PLoS ONE, 2022;17(1):e0262769. 2022 [PMID: 35077502] (IHC, Mouse) IHC Mouse
J Falero-Per, N Sheibani, CM Sorenson Bim expression modulates the pro-inflammatory phenotype of retinal astroglial cells PLoS ONE, 2020;15(5):e0232779. 2020 [PMID: 32365083] (Flow Cytometry, Mouse) Flow Cytometry Mouse
J Falero-Per, YS Song, Y Zhao, L Teixeira, CM Sorenson, N Sheibani Cyp1b1 expression impacts the angiogenic and inflammatory properties of liver sinusoidal endothelial cells PLoS ONE, 2018;13(10):e0206756. 2018 [PMID: 30372497] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Yang Y, Ahn Y, Chen Y, Tan X, Guo L, Gibbons D, Ungewiss C, Peng D, Liu X, Lin S, Thilaganathan N, Wistuba I, Rodriguez-Canales J, McLendon G, Creighton C, Kurie J ZEB1 sensitizes lung adenocarcinoma to metastasis suppression by PI3K antagonism. J Clin Invest, 2014;124(6):2696-708. 2014 [PMID: 24762440] (Western Blot, Mouse) Western Blot Mouse
Mir SU, Jin L, Craven RJ Neutrophil gelatinase-associated lipocalin (NGAL) expression is dependent on the tumor-associated sigma-2 receptor S2RPgrmc1. J. Biol. Chem., 2012;287(18):14494-501. 2012 [PMID: 22418433] (Western Blot, Human) Western Blot Human
Suryawanshi A, Mulik S, Sharma S Ocular neovascularization caused by herpes simplex virus type 1 infection results from breakdown of binding between vascular endothelial growth factor A and its soluble receptor. J. Immunol., 2011;186(6):3653-65. 2011 [PMID: 21325621] (Western Blot, Mouse) Western Blot Mouse
Isayeva T, Chanda D, Kallman L, Eltoum IE, Ponnazhagan S Effects of sustained antiangiogenic therapy in multistage prostate cancer in TRAMP model. Cancer Res., 2007;67(12):5789-97. 2007 [PMID: 17575146] (IHC-P, Mouse) IHC-P Mouse

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Product General Protocols

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Bioinformatics

Gene Symbol FLT1
Uniprot