VEGFR1/Flt-1 Antibody (141515) [Unconjugated]

Product Discontinued

VEGFR1/Flt-1 Antibody (141515) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse VEGFR1
  Ser27-Glu759
  Accession # P35969
• Specificity: Detects mouse VEGFR1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human VEGFR1, recombinant mouse (rm) VEGFR2, or rmVEGFR3 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: FLT1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGFR1/Flt-1 Antibody (141515) [Unconjugated]

• EC 2.7.10
• EC 2.7.10.1
• FLT
• FLT1
• Flt-1
• Fms-like tyrosine kinase 1
• fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor)
• FRT
• Tyrosine-protein kinase FRT
• Tyrosine-protein kinase receptor FLT
• vascular endothelial growth factor receptor 1
• Vascular permeability factor receptor
• VEGF R1
• VEGFR1
• VEGFR-1

Background

VEGFR1 (also called Flt-1) is one of the five receptor tyrosine kinases (RTKs) (VEGFR1, KDR/Flk-1, Flt-4, Tie-1, and Tek/Tie-2) whose expression is almost exclusively restricted to the endothelial cells. Tie-1 and Tie-2/Tek define a class of RTKs containing two immunoglobulin-like domains, three EGF homology domains and three fibronectin type III domains in their extracellular regions. VEGFR1/Flt-1, VEGFR2/KDR/Flk-1, VEGFR3/Flt-4 are members of the class III subfamily of RTKs containing seven immunoglobulin-like repeats in their extracellular domains. All five RTKs are likely to play central roles in vasculogenesis and angiogenesis.

Full length mouse VEGFR1 mRNA encodes a 1333 amino acid (aa) residue precursor with a 22 aa residue signal peptide. Mature VEGFR1 is composed of a 737 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 552 aa residue cytoplasmic domain. As a result of alternative splicing of the mRNA, a cDNA encoding a truncated form of VEGFR1, lacking the seventh immunoglobulin-like domain, the transmembrane and intracellular domains, has been cloned. The recombinant soluble VEGFR1/Fc chimera binds VEGF and PlGF with high affinity and is a potent VEGF antagonist.

1. He, Y. et al. (1999) Molecular Endocrinology 13:537.
2. Ferrara, N. and T. Davis-Smyth (1997) Endocrine Reviews 8:4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VEGFR1/Flt-1 Antibody (MAB471)(13)

Publications using MAB471

• J Sarkar, Y Luo, Q Zhou, E Ivakhnitsk, D Lara, E Katz, MG Sun, V Guaiquil, M Rosenblatt VEGF receptor heterodimers and homodimers are differentially expressed in neuronal and endothelial cell types PLoS ONE, 2022-07-21;17(7):e0269818. 2022-07-21 [PMID: 35862373] (ICC, Immunoprecipitation, Western Blot, Human, Rat)
• AX Xie, N Iguchi, TC Clarkson, AP Malykhina Pharmacogenetic inhibition of lumbosacral sensory neurons alleviates visceral hypersensitivity in a mouse model of chronic pelvic pain PLoS ONE, 2022-01-25;17(1):e0262769. 2022-01-25 [PMID: 35077502] (IHC, Mouse)
• J Falero-Per, N Sheibani, CM Sorenson Bim expression modulates the pro-inflammatory phenotype of retinal astroglial cells PLoS ONE, 2020-05-04;15(5):e0232779. 2020-05-04 [PMID: 32365083] (Flow Cytometry, Mouse)
• Nasim Jamali, Yong‐Seok Song, Christine M. Sorenson, Nader Sheibani 1,25(OH)2D3 regulates the proangiogenic activity of pericyte through VDR-mediated modulation of VEGF production and signaling of VEGF and PDGF receptors FASEB BioAdvances 2019-08-10 [PMID: 31396585]
• J Falero-Per, YS Song, Y Zhao, L Teixeira, CM Sorenson, N Sheibani Cyp1b1 expression impacts the angiogenic and inflammatory properties of liver sinusoidal endothelial cells PLoS ONE, 2018-10-29;13(10):e0206756. 2018-10-29 [PMID: 30372497] (Flow Cytometry, Mouse)
• Deepitha Selvaraj, Vijayan Gangadharan, Christoph W. Michalski, Martina Kurejova, Sebastian Stösser, Kshitij Srivastava et al. A Functional Role for VEGFR1 Expressed in Peripheral Sensory Neurons in Cancer Pain Cancer Cell 2015-06-08 [PMID: 26058077]
• Wen Wen Xu, Bin Li, Alfred K Y Lam, Sai Wah Tsao, Simon Y K Law, Kwok Wah Chan et al. Targeting VEGFR1- and VEGFR2-expressing non-tumor cells is essential for esophageal cancer therapy Oncotarget 2015-01-30 [PMID: 25595897] (Western Blot, Mouse)
• Yang Y, Ahn Y, Chen Y, Tan X, Guo L, Gibbons D, Ungewiss C, Peng D, Liu X, Lin S, Thilaganathan N, Wistuba I, Rodriguez-Canales J, McLendon G, Creighton C, Kurie J ZEB1 sensitizes lung adenocarcinoma to metastasis suppression by PI3K antagonism. J Clin Invest, 2014-04-24;124(6):2696-708. 2014-04-24 [PMID: 24762440] (Western Blot, Mouse)
• Anil Zechariah, Ayman ElAli, Thorsten R. Doeppner, Fengyan Jin, Mohammad R. Hasan, Iris Helfrich et al. Vascular Endothelial Growth Factor Promotes Pericyte Coverage of Brain Capillaries, Improves Cerebral Blood Flow During Subsequent Focal Cerebral Ischemia, and Preserves the Metabolic Penumbra Stroke 2013-06-01 [PMID: 23632977]
• Mir SU, Jin L, Craven RJ Neutrophil gelatinase-associated lipocalin (NGAL) expression is dependent on the tumor-associated sigma-2 receptor S2RPgrmc1. J. Biol. Chem., 2012-03-14;287(18):14494-501. 2012-03-14 [PMID: 22418433] (Western Blot, Human)
• Suryawanshi A, Mulik S, Sharma S Ocular neovascularization caused by herpes simplex virus type 1 infection results from breakdown of binding between vascular endothelial growth factor A and its soluble receptor. J. Immunol., 2011-02-16;186(6):3653-65. 2011-02-16 [PMID: 21325621] (Western Blot, Mouse)
• Anca Rosu, Ciurea Ciurea, Simionescu Simionescu, Margaritescu Margaritescu, Musetescu Musetescu, Ciurea Ciurea et al. The study of angiogenesis in early rheumatoid arthritis – clinical, immunohistochemical and immunological correlations J Med Life 2008-08-15 [PMID: 20108506] (Immunohistochemistry, Human)
• Isayeva T, Chanda D, Kallman L, Eltoum IE, Ponnazhagan S Effects of sustained antiangiogenic therapy in multistage prostate cancer in TRAMP model. Cancer Res., 2007-06-15;67(12):5789-97. 2007-06-15 [PMID: 17575146] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: FLT1
• Uniprot:
  • P35969()