VEGFR1/Flt-1 Antibody (141515) [Unconjugated]

• Reactivity: Mu
• Applications: WB, IHC
• Clone: 141515
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

VEGFR1/Flt-1 Antibody (141515) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse VEGFR1
  Ser27-Glu759
  Accession # P35969
• Specificity: Detects mouse VEGFR1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human VEGFR1, recombinant mouse (rm) VEGFR2, or rmVEGFR3 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: FLT1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGFR1/Flt-1 Antibody (141515) [Unconjugated]

• EC 2.7.10
• EC 2.7.10.1
• FLT
• FLT1
• Flt-1
• Fms-like tyrosine kinase 1
• fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor)
• FRT
• Tyrosine-protein kinase FRT
• Tyrosine-protein kinase receptor FLT
• vascular endothelial growth factor receptor 1
• Vascular permeability factor receptor
• VEGF R1
• VEGFR1
• VEGFR-1

Background

VEGFR1 (also called Flt-1) is one of the five receptor tyrosine kinases (RTKs) (VEGFR1, KDR/Flk-1, Flt-4, Tie-1, and Tek/Tie-2) whose expression is almost exclusively restricted to the endothelial cells. Tie-1 and Tie-2/Tek define a class of RTKs containing two immunoglobulin-like domains, three EGF homology domains and three fibronectin type III domains in their extracellular regions. VEGFR1/Flt-1, VEGFR2/KDR/Flk-1, VEGFR3/Flt-4 are members of the class III subfamily of RTKs containing seven immunoglobulin-like repeats in their extracellular domains. All five RTKs are likely to play central roles in vasculogenesis and angiogenesis.

Full length mouse VEGFR1 mRNA encodes a 1333 amino acid (aa) residue precursor with a 22 aa residue signal peptide. Mature VEGFR1 is composed of a 737 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 552 aa residue cytoplasmic domain. As a result of alternative splicing of the mRNA, a cDNA encoding a truncated form of VEGFR1, lacking the seventh immunoglobulin-like domain, the transmembrane and intracellular domains, has been cloned. The recombinant soluble VEGFR1/Fc chimera binds VEGF and PlGF with high affinity and is a potent VEGF antagonist.

1. He, Y. et al. (1999) Molecular Endocrinology 13:537.
2. Ferrara, N. and T. Davis-Smyth (1997) Endocrine Reviews 8:4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VEGFR1/Flt-1 Antibody (MAB471)(8)

Publications using MAB471

• J Sarkar, Y Luo, Q Zhou, E Ivakhnitsk, D Lara, E Katz, MG Sun, V Guaiquil, M Rosenblatt VEGF receptor heterodimers and homodimers are differentially expressed in neuronal and endothelial cell types PLoS ONE, 2022;17(7):e0269818. 2022 [PMID: 35862373] (Immunoprecipitation, Human)
• AX Xie, N Iguchi, TC Clarkson, AP Malykhina Pharmacogenetic inhibition of lumbosacral sensory neurons alleviates visceral hypersensitivity in a mouse model of chronic pelvic pain PLoS ONE, 2022;17(1):e0262769. 2022 [PMID: 35077502] (IHC, Mouse)
• J Falero-Per, N Sheibani, CM Sorenson Bim expression modulates the pro-inflammatory phenotype of retinal astroglial cells PLoS ONE, 2020;15(5):e0232779. 2020 [PMID: 32365083] (Flow Cytometry, Mouse)
• J Falero-Per, YS Song, Y Zhao, L Teixeira, CM Sorenson, N Sheibani Cyp1b1 expression impacts the angiogenic and inflammatory properties of liver sinusoidal endothelial cells PLoS ONE, 2018;13(10):e0206756. 2018 [PMID: 30372497] (Flow Cytometry, Mouse)
• Yang Y, Ahn Y, Chen Y, Tan X, Guo L, Gibbons D, Ungewiss C, Peng D, Liu X, Lin S, Thilaganathan N, Wistuba I, Rodriguez-Canales J, McLendon G, Creighton C, Kurie J ZEB1 sensitizes lung adenocarcinoma to metastasis suppression by PI3K antagonism. J Clin Invest, 2014;124(6):2696-708. 2014 [PMID: 24762440] (Western Blot, Mouse)
• Mir SU, Jin L, Craven RJ Neutrophil gelatinase-associated lipocalin (NGAL) expression is dependent on the tumor-associated sigma-2 receptor S2RPgrmc1. J. Biol. Chem., 2012;287(18):14494-501. 2012 [PMID: 22418433] (Western Blot, Human)
• Suryawanshi A, Mulik S, Sharma S Ocular neovascularization caused by herpes simplex virus type 1 infection results from breakdown of binding between vascular endothelial growth factor A and its soluble receptor. J. Immunol., 2011;186(6):3653-65. 2011 [PMID: 21325621] (Western Blot, Mouse)
• Isayeva T, Chanda D, Kallman L, Eltoum IE, Ponnazhagan S Effects of sustained antiangiogenic therapy in multistage prostate cancer in TRAMP model. Cancer Res., 2007;67(12):5789-97. 2007 [PMID: 17575146] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: FLT1
• Uniprot:
  • P35969()