VEGF-B Antibody [Alexa Fluor® 594]

• Reactivity: Mu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 594

VEGF-B Antibody [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant mouse VEGF-B₁₈₆
  Pro22-Ala107
  Accession # P49766
• Specificity: Detects mouse VEGF-B₁₆₇ and mouse VEGF-B₁₈₆ in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with rhVEGF-B₁₈₆ is observed, 15% cross-reactivity with rhVEGF-B₁₆₇
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: VEGFB
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGF-B Antibody [Alexa Fluor® 594]

• vascular endothelial growth factor B
• VEGFB
• VEGF-B
• VEGF-related factor
• VRFVEGFL

Background

Vascular endothelial growth factor B (VEGF-B; also known as VFR) is a member of the VEGF-PDGF supergene family of growth factor molecules (1 - 4). Five mouse members have been identified, including VEGF-A, -B, -C, -D, and PlGF(-2) (1, 5). VEGF family members are disulfide-linked homo- and heterodimeric proteins that are important regulators of vasculogenesis and lymphangiogenesis. Mouse VEGF-B has two isoforms, a 32 kDa single chain and a 21 kDa single chain form (6, 7). The long form (VEGF-B₁₈₆) is 207 amino acids (aa) in length, with a 21 aa signal sequence and a 186 aa mature region. The short form (VEGF-B₁₆₇) is 188 aa in length, with a 21 aa signal sequence and a 167 aa mature segment. Each mature isoform shows the same N-terminal 94 aa that contains a cysteine knot VEGF homology domain (6 - 8). VEGF-B₁₈₆ is O-glycosylated; VEGF-B₁₆₇ is not. VEGF-B₁₆₇ binds heparin; VEGF-B₁₈₆ does not. Thus, VEGF-B₁₈₆ is secreted and freely diffusible in tissues (7). However, the VEGF-B₁₆₇ isoform is the predominant form in tissue (9). Mouse VEGF-B₁₈₆ is 93% and 87% aa identical to bovine and human VEGF-B₁₈₆, respectively; mouse VEGF-B₁₆₇ is 90% and 88% aa identical to bovine and human VEGF-B₁₆₇, respectively . The mouse VEGF-B₁₆₇ homodimer is 42 kDa in size, while the VEGF-B₁₈₆ homodimer is 62 kDa in size. Unlike VEGF₁₆₇, VEGF-B₁₈₆ undergoes proteolytic processing that creates a partially processed 48 kDa homodimer and a fully processed 32 kDa homodimer. Processing appears to occur at Arg127 of the mature form (10). Both forms of VEGF-B can heterodimerize with VEGF (7). Both VEGF-B isoforms bind to VEGF receptor 1 (VEGF R1), but not VEGF R2 or VEGF R3 (11). VEGF-B₁₆₇ also binds neuropilin-1, but only the 127 aa processed form of VEGF-B₁₈₆ binds neuropilin-1 (10). As a dimer, full length VEGF-B₁₈₆ does not interact with neuropilin-1, while any dimer that contains the processed VEGF-B₁₂₇ subunit will interact with neuropilin-1 (10). The importance of differential neuropilin binding is unclear. VEGF-B deficient mice display an atrial conduction deficit (12). On endothelial cells, ligation of VEGF R1 by VEGF-B has been shown to regulate the expression and activity of urokinase type plasminogen activator and plasminogen activator inhibitor 1 (11).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VEGFB