Recombinant Rat VEGF 164 Protein

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications Bioactivity
Concentration
LYOPH

Order Details

Recombinant Rat VEGF 164 Protein Summary

Description
A disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains corresponding to VEGF 164. As a result of glycosylation, it migrates to at least two bands with molecular weights ranging from 257 kDa in SDS-PAGE under reducing conditions Source: Yeast

Amino Acid Sequence: MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

Details of Functionality
VEGF 164 protein is fully biologically active when compared to standard. Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 0.75-3.75 ng/mL.
Source
Yeast
Protein/Peptide Type
Biologically Active Protein
Gene
VEGFA
Purity
>95%, by SDS-PAGE
Endotoxin Note
Less than 0.1 EU/ug of VEGF 164 as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Application Notes
As a result of glycosylation, it migrates to at least two bands with molecular weights ranging from 25.7 kDa in SDS-PAGE under reducing conditions.
Theoretical MW
25.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol VEGFA