Recombinant Rat VEGF 164 Protein

• Reactivity: Rt
• Applications: Bioactivity
• Concentration: LYOPH

Recombinant Rat VEGF 164 Protein Summary

• Description: A disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains corresponding to VEGF 164. As a result of glycosylation, it migrates to at least two bands with molecular weights ranging from 257 kDa in SDS-PAGE under reducing conditions Source: Yeast

  Amino Acid Sequence: MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

• Details of Functionality: VEGF 164 protein is fully biologically active when compared to standard. Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 0.75-3.75 ng/mL.
• Source: Yeast
• Protein/Peptide Type: Recombinant Protein
• Gene: VEGFA
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: Less than 0.1 EU/ug of VEGF 164 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Application Notes: As a result of glycosylation, it migrates to at least two bands with molecular weights ranging from 25.7 kDa in SDS-PAGE under reducing conditions.
• Theoretical MW: 25.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: VEGFA