VE-Cadherin [p Tyr731] Antibody

Product Discontinued

VE-Cadherin [p Tyr731] Antibody Summary

• Immunogen: Synthetic phosphopeptide derived from human VE-Cadherin around the phosphorylation site of Tyrosine 731.
• Modification: p Tyr731
• Marker: Endothelial Cell Marker
• Specificity: p-VE-Cadherin (Y731) pAb detects endogenous levels of VE-Cadherin protein only when phosphorylated at Y731
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDH5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of p-VE-Cadherin (Y731) pAb in extracts from HepG2 cells treated with Na3VO4Immunohistochemistry (IHC) analyzes of p-VE-Cadherin (Y731) pAb in paraffin-embedded human brain tissue.
• Theoretical MW: 130 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for VE-Cadherin [p Tyr731] Antibody

• 7B4 antigen
• 7B4
• cadherin 5, type 2 (vascular endothelium)
• cadherin 5, type 2, VE-cadherin (vascular epithelium)
• Cadherin-5
• CD144 antigen
• CD144
• CDH5
• endothelial-specific cadherin
• FLJ17376
• Vascular endothelial cadherin
• VECadherin
• VE-Cadherin

Background

Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha catenin forming a link to the cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VE-Cadherin Antibody (NBP1-51393)(4)

Publications using NBP1-51393

• Gliemann L, Rytter N, Piil P et al. The Endothelial Mechanotransduction Protein Platelet Endothelial Cell Adhesion Molecule-1 Is Influenced by Aging and Exercise Training in Human Skeletal Muscle. Front Physiol 2018-12-18 [PMID: 30618819] (WB, Human)
• Gliemann L, Rytter N, Lindskrog M et al. Endothelial mechanotransduction proteins and vascular function are altered by dietary sucrose supplementation in healthy young male subjects J. Physiol. (Lond.) 2017-08-15 [PMID: 28620941] (WB, Human)
• Cao HJ, Zheng LZ, Wang N et al. Src blockage by siRNA inhibits VEGF-induced vascular hyperpemeability and osteoclast activity - an in vitro mechanism study for preventing destructive repair of osteonecrosis. Bone 2015-05-01 [PMID: 25554601] (WB, Human)
• Wessel F, Winderlich M, Holm M et al. Leukocyte extravasation and vascular permeability are each controlled in vivo by different tyrosine residues of VE-cadherin. Nat. Immunol. 2014-02-19 [PMID: 24487320] (WB, Mouse)

Bioinformatics

• Gene Symbol: CDH5
• Entrez:
  • 1003(Human)
  • 12562(Mouse)
• Uniprot:
  • P33151()