VE-Cadherin Antibody (BV9) - Azide Free

Immunocytochemistry/Immunofluorescence: VE Cadherin Antibody (BV9) [NB600-1409] - Endothelial cells were fixed with 4% PAF (15 min, room temp), and then permeabilized with 0.5% TritionX-100 (3 min, room temperature). Cells were incubated with a final concentration of 10mg/ml BV9. Secondary detection was performed with anti-mouse Alexa-Fluor 647 and counterstained using DAPI.

Flow Cytometry: VE Cadherin Antibody (BV9) [NB600-1409] - HUVEC cells were stained with antibody BV9 in PBS/0.1% saponin for 1h at 4 degrees C.(Black- no stain, Red- isotype control, Blue-HM2032-BV9)

• Reactivity: Hu
• Applications: WB, ELISA, Flow, Func, ICC/IF, IHC, IP
• Clone: BV9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide Free
• Concentration: 0.1 mg/ml

VE-Cadherin Antibody (BV9) - Azide Free Summary

• Additional Information: Clone BV9 was used by HLDA to establish CD designation.
• Immunogen: The antibody recognizes the extracellular domain of the protein.
• Localization: Intercellular junctions
• Marker: Endothelial Cell Marker
• Specificity: This monoclonal antibody (subclass IgG2a) binds to human VE-cadherin. Human vascular endothelium cadherin is an endothelium-specific cadherin localized at the intercellular junctions.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDH5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:100
  • Functional 10-50 ug/ml
  • Immunoassay
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:10-1:100
• Control:

  E-Cadherin Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS with 0.1% BSA
• Preservative: No Preservative
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for VE-Cadherin Antibody (BV9) - Azide Free

• 7B4 antigen
• 7B4
• cadherin 5, type 2 (vascular endothelium)
• cadherin 5, type 2, VE-cadherin (vascular epithelium)
• Cadherin-5
• CD144 antigen
• CD144
• CDH5
• endothelial-specific cadherin
• FLJ17376
• Vascular endothelial cadherin
• VECadherin
• VE-Cadherin

Background

Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha catenin forming a link to the cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VE-Cadherin Antibody (NB600-1409)(7)

Publications using NB600-1409

• Wang H, Zhang X, Liu Y et al. Diosmetin-7-O-?-D-glucopyranoside suppresses endothelial-mesenchymal transformation through endoplasmic reticulum stress in cardiac fibrosis Clinical and experimental pharmacology & physiology 2023-07-10 [PMID: 37430476]
• Tryfonos A, Mills J, Green DJ et al. Association between atherogenic risk-modulating proteins and endothelium-dependent flow-mediated dilation in coronary artery disease patients European Journal of Applied Physiology 2023-02-01 [PMID: 36305972] (Western Blot, Human)
• Depanwita Saha, Debarpan Mitra, Neyaz Alam, Sagar Sen, Saunak Mitra Mustafi, Pradip K Majumder, Biswanath Majumder, Nabendu Murmu Lupeol and Paclitaxel cooperate in hindering hypoxia induced vasculogenic mimicry via suppression of HIF-1 alpha -EphA2-Laminin-5 gamma 2 network in human oral cancer. Journal of cell communication and signaling 2023-09-01 [PMID: 36063341]
• Mylvaganam S, Plumb J, Yusuf B et al. The spectrin cytoskeleton integrates endothelial mechanoresponses Nature cell biology 2022-07-11 [PMID: 35817960] (ICC/IF, Human)
  
  Details:
  ICC/IF Dilution: 4.5 ug ml–1, WB Dilution: 1:1000
• Tryfonos A, Rasoul D, Sadler D et al. Elevated shear rate-induced by exercise increases eNOS ser1177 but not PECAM-1 Tyr713 phosphorylation in human conduit artery endothelial cells European journal of sport science 2022-02-23 [PMID: 35195045] (ICC/IF, Human)
• Sarveswaran K, Kurz V, Dong Z et al. Synthetic Capillaries to Control Microscopic Blood Flow Sci Rep 2016-02-24 [PMID: 26905751] (ICC/IF, Human)
• Evangelista K, Franco R, Schwab A. Leptospira interrogans Binds to Cadherins. PLoS Negl Trop Dis 2014-02-05 [PMID: 24498454] (WB, Human)

Bioinformatics

• Gene Symbol: CDH5
• Entrez:
  • 1003(Human)
• Uniprot:
  • P33151()