![Western Blot: VCIP135/VCPIP1 Antibody [NBP1-49939] - Detection of Human VCIP135/VCPIP1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-VCIP135 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. VCIP135 was also immunoprecipitated by rabbit anti-VCIP135 antibody which recognizes a downstream epitope. For blotting immunoprecipitated VCIP135 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/VCIP135-VCPIP1-Antibody-Western-Blot-NBP1-49939-img0002.jpg "Western Blot: VCIP135/VCPIP1 Antibody [NBP1-49939] - Detection of Human VCIP135/VCPIP1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-VCIP135 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. VCIP135 was also immunoprecipitated by rabbit anti-VCIP135 antibody which recognizes a downstream epitope. For blotting immunoprecipitated VCIP135 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | Novus Biologicals Rabbit VCIP135/VCPIP1 Antibody (NBP1-49939) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-VCIP135/VCPIP1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | The immunogen for this product maps to a region between residue 1100 and 1150 of human Valosin-Containing Protein (p97)/p47 Complex-Interacting Protein p135 using the numbering given in entry NP_079330.2 (GeneID 80124). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | VCPIP1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP1-49939 | Applications | Species |
|---|---|---|
| Li JT, Yang H, Lei MZ et al. Dietary folate drives methionine metabolism to promote cancer development by stabilizing MAT IIA Signal transduction and targeted therapy 2022-06-22 [PMID: 35729157] (IHC-P, Human) | IHC-P | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for VCIP135/VCPIP1 Antibody (NBP1-49939)Find related products by research area.
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| Gene Symbol | VCPIP1 |
![Immunohistochemistry-Paraffin: VCIP135/VCPIP1 Antibody [NBP1-49939] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- VCIP135 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/VCIP135-VCPIP1-Antibody-Immunohistochemistry-Paraffin-NBP1-49939-img0009.jpg "Immunohistochemistry-Paraffin: VCIP135/VCPIP1 Antibody [NBP1-49939] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- VCIP135 used at a dilution of 1:200 (1ug/ml). Detection: DAB")
![Immunohistochemistry-Paraffin: VCIP135/VCPIP1 Antibody [NBP1-49939] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- VCIP135 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/VCIP135-VCPIP1-Antibody-Immunohistochemistry-Paraffin-NBP1-49939-img0010.jpg "Immunohistochemistry-Paraffin: VCIP135/VCPIP1 Antibody [NBP1-49939] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- VCIP135 used at a dilution of 1:200 (1ug/ml). Detection: DAB")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")