PSMF1 Antibody - Isoform 1

Images

 
Western Blot: PSMF1 Antibody - Isoform 1 [NB100-1178] - Staining of KNRK (A), NIH3T3 (B) with antibody at 1 ug/mL and Neuro2a (C) cell lysate with 0.3 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: PSMF1 Antibody - Isoform 1 [NB100-1178] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) ...read more
Western Blot: PSMF1 Antibody - Isoform 1 [NB100-1178] - Staining of HEK293 (A), K562 (B) with antibody at 0.3 ug/mL and LNCaP (C) cell lysate with 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, Flow-IC, ICC/IF, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PSMF1 Antibody - Isoform 1 Summary

Immunogen
Peptide with sequence C-DHLPPPGYDDMYL corresponding to C-Terminus according to NP_006805.2.
Specificity
This antibody is expected to recognize isoform 1 (NP_006805.2 and NP_848693.2).
Predicted Species
Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PSMF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow (Intracellular)
  • Immunocytochemistry/Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/mL
Application Notes
Use in Flow-intracellular reported in scientific literature (PMID: 19015396).
Control
Human Kidney Whole Tissue Lysate (Adult Whole Normal)
PSMF1 Overexpression Lysate
Publications
Read Publications using NB100-1178.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PSMF1 Antibody - Isoform 1

  • hPI31
  • PI31
  • proteasome (prosome, macropain) inhibitor subunit 1 (PI31)
  • proteasome inhibitor hP131 subunit
  • proteasome inhibitor PI31 subunit

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PSMF1 Antibody (NB100-1178)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PSMF1
Entrez
Uniprot