PSMF1 Antibody - Isoform 1

Western Blot: PSMF1 Antibody - Isoform 1 [NB100-1178] - Staining of KNRK (A), NIH3T3 (B) with antibody at 1 ug/mL and Neuro2a (C) cell lysate with 0.3 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: PSMF1 Antibody - Isoform 1 [NB100-1178] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Western Blot: PSMF1 Antibody - Isoform 1 [NB100-1178] - Staining of HEK293 (A), K562 (B) with antibody at 0.3 ug/mL and LNCaP (C) cell lysate with 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PSMF1 Antibody - Isoform 1 Summary

• Immunogen: Peptide with sequence C-DHLPPPGYDDMYL corresponding to C-Terminus according to NP_006805.2.
• Specificity: This antibody is expected to recognize isoform 1 (NP_006805.2 and NP_848693.2).
• Predicted Species: Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PSMF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular)
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/mL
• Application Notes: Use in Flow-intracellular reported in scientific literature (PMID: 19015396).
• Control:

  Human Kidney Whole Tissue Lysate (Adult Whole Normal)
  PSMF1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PSMF1 Antibody - Isoform 1

• hPI31
• PI31
• proteasome (prosome, macropain) inhibitor subunit 1 (PI31)
• proteasome inhibitor hP131 subunit
• proteasome inhibitor PI31 subunit

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PSMF1 Antibody (NB100-1178)(2)

Publications using NB100-1178

• Sadaka C, Marloie-Provost MA, Soumelis V, Benaroch P. Developmental regulation of MHC II expression and transport in human plasmacytoid-derived dendritic cells. Blood. 2009-03-05 [PMID: 19015396] (Flow Cytometry Control)
  
  Details:
  Human pDC cells were pre-incubated with IMG-2000, Fig. 7.
• McCutchen-Maloney SL, Matsuda K, Shimbara N et al. cDNA cloning, expression, and functional characterization of PI31, a proline-rich inhibitor of the proteasome. J Biol Chem 2000-06-16 [PMID: 10764772]

Bioinformatics

• Gene Symbol: PSMF1
• Entrez:
  • 9491(Human)
  • 228769(Mouse)
• Uniprot:
  • NP_006805(Human)