VCIP135/VCPIP1 Antibody - BSA Free

Western Blot: VCIP135/VCPIP1 Antibody [NBP1-32663] - Wild-type (WT) and VCPIP1 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with VCPIP1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: VCIP135/VCPIP1 Antibody [NBP1-32663] - HeLa cells were fixed in -20C 100% MeOH for 5 min. Green: VCPIP1 protein stained by VCPIP1 antibody, C-term diluted at 1:500. Blue: Hoechst 33343 staining.

Immunohistochemistry-Paraffin: VCIP135/VCPIP1 Antibody [NBP1-32663] - Paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution.

Western Blot: VCIP135/VCPIP1 Antibody [NBP1-32663] - A: Mouse brain.

Western Blot: VCIP135/VCPIP1 Antibody [NBP1-32663] - A: H1299.

• Reactivity: Hu, Mu, RM
• Applications: WB, ICC/IF, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

VCIP135/VCPIP1 Antibody - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit VCIP135/VCPIP1 Antibody - BSA Free (NBP1-32663) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human VCIP135/VCPIP1. The exact sequence is proprietary.
• Localization: Endoplasmic reticulum, Golgi apparatus, Golgi stack
• Predicted Species: Rhesus Macaque (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VCPIP1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 134 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for VCIP135/VCPIP1 Antibody - BSA Free

• DKFZp686G038
• DUBA3
• EC 3.4.22.-
• FLJ23132
• KIAA1850FLJ60694
• valosin containing protein (p97)/p47 complex interacting protein 1
• valosin-containing protein (p97)/p47 complex-interacting protein p135
• Valosin-containing protein p97/p47 complex-interacting protein 1
• Valosin-containing protein p97/p47 complex-interacting protein p135
• VCIP135
• VCIP135deubiquitinating protein VCIP135
• VCPIP1 VCIP135
• VCPIP1

Background

VCPIP1 - valosin containing protein (p97)/p47 complex interacting protein 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: VCPIP1