Vasohibin Antibody

Images

 
Western Blot: Vasohibin Antibody [NBP1-76579] - Western blot analysis of VASH1 in human brain tissue lysate with VASH1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: Vasohibin Antibody [NBP1-76579] - Immunofluorescence of VASH1 in mouse brain tissue with VASH1 antibody at 20 ug/mL.
Immunohistochemistry: Vasohibin Antibody [NBP1-76579] - Immunohistochemistry of VASH1 in mouse brain tissue with VASH1 antibody at 5 ug/mL.
Western Blot: Vasohibin Antibody [NBP1-76579] - Human brain tissue lysate with VASH1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Vasohibin Antibody Summary

Immunogen
Antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human VASH1. The immunogen is located within amino acids 290 - 340 of VASH1.
Specificity
VASH1 antibody is predicted to not cross-react with other vasohibin protein family members. At least two isoforms are known to exist; this antibody will recognize only the long isoform.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VASH1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
VASH1 antibody can be used for detection of VASH1 by Western blot at 1 - 2 mg/mL.
Control Peptide
Vasohibin Peptide (NBP1-76579PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Vasohibin Antibody

  • KIAA1036
  • KIAA1036vasohibin-1
  • VASH
  • VASH1
  • vasohibin 1
  • Vasohibin

Background

VASH1 was originally identified as an endothelium-derived vascular endothelial growth factor (VEGF)-inducible angiogenesis inhibitor that acts in a negative feedback manner. VASH1 mRNA is expressed brain, heart, kidney and placenta in the adult, with robust expression in various organs during embryonic development. VASH1 expression in tumor cells and tumor vasculature is silenced by methylation by increased expression of Zeste homolog 2 (EZH2). The increase of EZH2 expression is induced by VEGF stimulation, leading to poor clinical prognosis. Recombinant adenovirus expressing VASH prevented tumor angiogenesis and inhibited tumor growth, suggesting that it may be a potentially valuable antitumor therapy in the clinic.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol VASH1
Entrez
Uniprot