Vasohibin Antibody - BSA Free

Western Blot: Vasohibin Antibody [NBP1-76579] - Western blot analysis of VASH1 in human brain tissue lysate with VASH1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.

Immunocytochemistry/ Immunofluorescence: Vasohibin Antibody - BSA Free [NBP1-76579] - Immunofluorescence of Vasohibin in mouse brain tissue with Vasohibin antibody at 20 ug/mL.

Immunohistochemistry: Vasohibin Antibody - BSA Free [NBP1-76579] - Immunohistochemistry of Vasohibin in mouse brain tissue with Vasohibin antibody at 5 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Vasohibin Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Vasohibin Antibody - BSA Free (NBP1-76579) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human VASH1. The immunogen is located within amino acids 290 - 340 of VASH1. Amino Acid Squence: KIGKGTGPPSPTKDRKKDV
• Specificity: VASH1 antibody is predicted to not cross-react with other vasohibin protein family members. At least two isoforms are known to exist; this antibody will recognize only the long isoform.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VASH1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1 ug/ml
• Control Peptide:

  Vasohibin Peptide (NBP1-76579PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Vasohibin Antibody - BSA Free

• KIAA1036
• KIAA1036vasohibin-1
• VASH
• VASH1
• vasohibin 1
• Vasohibin

Background

VASH1 was originally identified as an endothelium-derived vascular endothelial growth factor (VEGF)-inducible angiogenesis inhibitor that acts in a negative feedback manner. VASH1 mRNA is expressed brain, heart, kidney and placenta in the adult, with robust expression in various organs during embryonic development. VASH1 expression in tumor cells and tumor vasculature is silenced by methylation by increased expression of Zeste homolog 2 (EZH2). The increase of EZH2 expression is induced by VEGF stimulation, leading to poor clinical prognosis. Recombinant adenovirus expressing VASH prevented tumor angiogenesis and inhibited tumor growth, suggesting that it may be a potentially valuable antitumor therapy in the clinic.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VASH1
• Uniprot:
  • NP_055724()