Human VAP-1/AOC3 ELISA Kit (Colorimetric)

Human VAP-1/AOC3 ELISA Kit [NBP1-91261] - Data for Human sVAP-1 Platinum ELISA

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum, plasma (EDTA, heparin), buffered solution, amniotic fluid
• Standard Curve Range: 31.3 - 2,000 pg/mL
• Sensitivity: 19 pg/mL

Human VAP-1/AOC3 ELISA Kit (Colorimetric) Summary

• Description: The human sVAP-1 ELISA is an enzyme-linked immunosorbent assay for quantitative detection.
• Specificity: This kit detects the soluble form of VAP-1. HAMA may interfere with assays utilizing murine monoclonal antibodies leading to both false positive and false negative results. Serum samples containing antibodies to murine immunoglobulins can still be analysed in such assays when murine immunoglobulins (serum, ascitic fluid, or monoclonal antibodies of irrelevant specificity) are added to the sample.
• Standard Curve Range: 31.3 - 2,000 pg/mL
• Sensitivity: 19 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 4.50%
• Intra-Assay: CV% < 3.00%
• Spike Recovery: The amount of endogenous human VAP-1 in unspiked serum was substracted from the spike values. The recovery ranged from 82% to 117% with an overall mean recovery of 96%.
• Sample Volume: 10 uL (1:100 prediluted)
• Kit Type: ELISA Kit (Colorimetric)
• Gene: AOC3

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human VAP-1/AOC3 ELISA Kit (Colorimetric)

• amine oxidase, copper containing 3 (vascular adhesion protein 1)
• AOC3
• Copper amine oxidase
• EC 1.4.3
• HPAO
• HPAOSSAO
• Semicarbazide-sensitive amine oxidase
• SSAO
• VAP1
• VAP-1
• VAP1EC 1.4.3.21
• VAP-1membrane primary amine oxidase
• Vascular adhesion protein 1

Background

Lymphocyte binding to vascular endothelium is a prerequisite for the move- ment of immune cells from the blood into lymphoid tissues and into sites of inflammation. Under inflammatory conditions, cell surface expression of VAP-1 (vascular adhesion protein-1) which is an endothelial sialoglycoprotein, is induced. VAP-1 is a type II transmembrane protein of 84.6 kDa with a single transmembrane domain and N and O-glycosylation sites in the extracellular domain. In vivo, VAP-1 exists predominantly as a 180 kDa homodimer and functions both as an enzyme (monoamine oxidase) and an adhesion molecule for lymphocytes. A circulating form of human Vascular Adhesion Protein-1 (sVAP-1) has been characterized. This sVAP-1 has been shown to be elevated in sera of patients with certain liver diseases and a correlation with the diagnosis of the patients was demonstrated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: AOC3