VAP-1/AOC3 Antibody (393112) [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB
• Clone: 393112
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

VAP-1/AOC3 Antibody (393112) [Alexa Fluor® 350] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VAP‑1/AOC3
  Gly27-Asn763
  Accession # Q16853
• Specificity: Detects human VAP‑1/AOC3 in direct ELISAs and Western blots. In Western blots, 50% cross-reactivity with recombinant mouse
  VAP‑1/AOC3 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VAP-1/AOC3 Antibody (393112) [Alexa Fluor® 350]

• amine oxidase, copper containing 3 (vascular adhesion protein 1)
• AOC3
• Copper amine oxidase
• EC 1.4.3
• HPAO
• HPAOSSAO
• Semicarbazide-sensitive amine oxidase
• SSAO
• VAP1
• VAP-1
• VAP1EC 1.4.3.21
• VAP-1membrane primary amine oxidase
• Vascular adhesion protein 1

Background

Vascular adhesion protein-1 (VAP-1), also called AOC3 (amine oxidase copper-containing 3) or SSAO (semicarbazide-sensitive amine oxidase), is a copper amine oxidase with a topaquinone cofactor. VAP-1 is a Type II integral membrane protein, but a soluble form of the enzyme is present in human serum, and its level increases in diabetes and some inflammatory liver diseases (1, 2). Human and mouse VAP-1 share 83% amino acid sequence identity. VAP-1 catalyzes the oxidative deamination of small primary amines such as methylamine, benzylamine, and aminoacetone in a reaction that produces an aldehyde, ammonia, and H₂O₂ (3). The enzyme is sensitive to inhibition by semicarbazide. VAP-1 expression is highest in the endothelium of lung, heart, and intestine, but low in tissues such as brain, spleen, kidney, and liver (4). VAP-1 vascular expression is regulated at sites of inflammation through its release from intracellular granules in which the protein is stored (5). The adhesive function of VAP-1 has been demonstrated in studies showing that the protein is important for the adherence of certain lymphocyte subtypes to inflamed endothelial tissues (6). VAP-1 mediated adhesion is involved in the process of leukocyte extravasation, an important feature of inflammatory responses. The role of VAP-1 amine oxidase activity in this process is not fully defined, but it appears to be carbohydrate-dependent (7). VAP-1 is considered to be a therapeutic target for diabetes, oxidative stress, and inflammatory diseases (8).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.