VAMP3/Cellubrevin Antibody (4G9) [Alexa Fluor® 405]

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, IP
• Clone: 4G9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

VAMP3/Cellubrevin Antibody (4G9) [Alexa Fluor® 405] Summary

• Immunogen: Recombinant human VAMP3/Cellubrevin (1-77aa) purified from E. coli
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: VAMP3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for VAMP3/Cellubrevin Antibody (4G9) [Alexa Fluor® 405]

• CEBCellubrevin
• cellubrevin
• SYB3
• Synaptobrevin-3
• VAMP-3
• vesicle-associated membrane protein 3 (cellubrevin)
• vesicle-associated membrane protein 3

Background

The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer®s disease, all VAMP isoform levels appear to be significantly reduced.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VAMP3/Cellubrevin Antibody (NBP1-97948AF405)(2)

Publications using NBP1-97948AF405

• Beckner ME, Conkright WR, Sahu A Et al. Utility of extracellular vesicles as a potential biological indicator of physiological resilience during military operational stress Physiol Rep 2022-04-04 [PMID: 35373929] (ICC/IF, Human)
  
  Details:
  Citation using the Alexa Fluor 700 version of this antibody.
• Conkright WR, Beckner ME, Sahu A et al. Men and Women Display Distinct Extracellular Vesicle Biomarker Signatures in Response to Military Operational Stress Journal of applied physiology (Bethesda, Md. : 1985) 2022-03-17 [PMID: 35297690]

Bioinformatics

• Gene Symbol: VAMP3