VAMP3/Cellubrevin Antibody (4G9) - BSA Free

Western Blot: VAMP3/Cellubrevin Antibody (4G9) [NBP1-97948] - The mouse brain tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human cellubrevin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Flow Cytometry: VAMP3/Cellubrevin Antibody (4G9) [NBP1-97948] - Analysis of Cellubrevin in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line)

Western Blot: VAMP3/Cellubrevin Antibody (4G9) [NBP1-97948] - Lane 1. 293T cell lysate Lane 2. Cellubrevin transfected 293T cell lysate.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, IP
• Clone: 4G9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

VAMP3/Cellubrevin Antibody (4G9) - BSA Free Summary

• Description: Novus Biologicals Mouse VAMP3/Cellubrevin Antibody (4G9) - BSA Free (NBP1-97948) is a monoclonal antibody validated for use in WB, ELISA, Flow, ICC/IF and IP. Anti-VAMP3/Cellubrevin Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human VAMP3/Cellubrevin (1-77aa) purified from E. coli
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: VAMP3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunoprecipitation
  • Western Blot 1:1000
• Application Notes: Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Use in immunoprecipitation reported in scientific literature (PMID 27183589).

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for VAMP3/Cellubrevin Antibody (4G9) - BSA Free

• CEBCellubrevin
• cellubrevin
• SYB3
• Synaptobrevin-3
• VAMP-3
• vesicle-associated membrane protein 3 (cellubrevin)
• vesicle-associated membrane protein 3

Background

Cellubrevin, also known as VAMP3, is a member of the Vamp/synaptobrevin family. Vamps, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. Cellubrevin is a vesicular soluble N-ethylmaleimide sensitive factor attachment protein receptor (v-SNARE) homologous to the neuronal synaptobrevins 1/2 and is also a substrate of tetanus neurotoxin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VAMP3/Cellubrevin Antibody (NBP1-97948)(3)

Publications using NBP1-97948

• Beckner ME, Conkright WR, Mi Q et al. Neuroendocrine, Inflammatory, and Extracellular Vesicle Responses During the Navy Special Warfare Screener Selection Course Physiological genomics 2022-06-13 [PMID: 35695270]
• Beckner ME, Conkright WR, Sahu A Et al. Utility of extracellular vesicles as a potential biological indicator of physiological resilience during military operational stress Physiol Rep 2022-04-04 [PMID: 35373929] (ICC/IF, Human)
  
  Details:
  Citation using the Alexa Fluor 700 version of this antibody.
• Martin-avila A, Medina-Tamayo J, Ibarra-Sanchez A et al. Protein Tyrosine kinase Fyn Regulates TLR4-Elicited Responses on Mast Cells Controlling the Function of a PP2A-PkCalpha/beta Signaling Node Leading to TNF Secretion. J. Immunol. 2016-05-11 [PMID: 27183589] (IP, Mouse)

Bioinformatics

• Gene Symbol: VAMP3
• Uniprot:
  • NP_004772(Human)