VAMP3/Cellubrevin Antibody (4G9)

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Western Blot: VAMP3/Cellubrevin Antibody (4G9) [NBP1-97948] - The mouse brain tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human cellubrevin antibody (1:1000). ...read more
Western Blot: VAMP3/Cellubrevin Antibody (4G9) [NBP1-97948] - Lane 1. 293T cell lysate Lane 2. Cellubrevin transfected 293T cell lysate.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow
Clone
4G9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

VAMP3/Cellubrevin Antibody (4G9) Summary

Immunogen
Recombinant human cellubrevin (1-77aa) purified from E. coli
Specificity
Anti-human VAMP3 mAb, clone k2A2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human cellubrevin protein.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
VAMP3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:100-1:2000
  • Flow Cytometry
Application Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000. Use in immunoprecipitation reported in scientific literature (PMID 27183589).
Publications
Read Publication using
NBP1-97948 in the following applications:

  • IP
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for VAMP3/Cellubrevin Antibody (4G9)

  • CEBCellubrevin
  • cellubrevin
  • SYB3
  • Synaptobrevin-3
  • VAMP-3
  • vesicle-associated membrane protein 3 (cellubrevin)
  • vesicle-associated membrane protein 3

Background

Cellubrevin, also known as VAMP3, is a member of the Vamp/synaptobrevin family. Vamps, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. Cellubrevin is a vesicular soluble N-ethylmaleimide sensitive factor attachment protein receptor (v-SNARE) homologous to the neuronal synaptobrevins 1/2 and is also a substrate of tetanus neurotoxin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VAMP3/Cellubrevin Antibody (NBP1-97948)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IP.


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Bioinformatics

Gene Symbol VAMP3
Uniprot