USP48 Antibody - BSA Free

Western Blot: USP48 Antibody [NBP3-38119] - Western blot analysis of various lysates using USP48 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

USP48 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit USP48 Antibody - BSA Free (NBP3-38119) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human USP48 (NP_115612.4).
  
  Sequence:
  MAPRLQLEKAAWRWAETVRPEEVSQEHIETAYRIWLEPCIRGVCRRNCKGNPNCLVGIGEHIWLGEIDENSFHNIDDPNCERRKKNSFVGLTNLGATCYVNTFLQVWFLNLELRQALYLCPSTCSDYMLGDGIQEEKDYEPQTICEHLQYLFALLQNSNRRYIDPSGFVKALGLDTGQQQDAQEFSKLFMSLLEDTLSKQKNPDVRNIVQQQFCGEYAYVTVCNQCGRESKLLSKFYELELNIQGHKQLTDCISEFLKEEKLEGDNRYFCENCQSKQNATRKIRLLSLPCTLNLQLMRFV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: USP48
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 119 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for USP48 Antibody - BSA Free

• Deubiquitinating enzyme 48
• DKFZp762M1713
• EC 3.1.2.15
• EC 3.4.19.12
• FLJ11328
• FLJ20103
• FLJ23054
• FLJ23277
• MGC132556
• MGC14879
• RAP1GA1
• synUSP
• ubiquitin carboxyl-terminal hydrolase 48
• ubiquitin specific peptidase 48
• ubiquitin specific protease 31
• ubiquitin specific protease 48
• ubiquitin thioesterase 48
• Ubiquitin thiolesterase 48
• Ubiquitin-specific-processing protease 48
• USP31
• USP48

Background

The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific peptidase 48 (USP48) is a member of the C19 peptidase family involved in the processing of polyubiquitin precursors as well as that of ubiquitinated proteins. USP48 is involved in the activation of NF-kappa B via its interaction with RELA and TRAF2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: USP48