DUSP5 Antibody (2F3)

Western Blot: DUSP5 Antibody (2F3) [H00001847-M04] - Analysis of DUSP5 expression in transfected 293T cell line by DUSP5 monoclonal antibody (M04), clone 2F3. Lane 1: DUSP5 transfected lysate (Predicted MW: 42.1 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: DUSP5 Antibody (2F3) [H00001847-M04] - Analysis of monoclonal antibody to DUSP5 on HeLa cell. Antibody concentration 10 ug/ml

Western Blot: DUSP5 Antibody (2F3) [H00001847-M04] - Western Blot analysis of DUSP5 expression in PC-12 ( Cat # L012V1 ).

Western Blot: DUSP5 Antibody (2F3) [H00001847-M04] - Analysis of DUSP5 expression in K-562 (Cat # L009V1).

ELISA: DUSP5 Antibody (2F3) [H00001847-M04] - Detection limit for recombinant GST tagged DUSP5 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, ICC/IF
• Clone: 2F3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

DUSP5 Antibody (2F3) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: DUSP5 (NP_004410, 286 a.a. ~ 384 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LKEAFDYIKQRRSMVSPNFGFMGQLLQYESEILPSTPNPQPPSCQGEAAGSSLIGHLQTLSPDMQGAYCTFPASVLAPVPTHSTVSELSRSPVATATSC
• Specificity: DUSP5 - dual specificity phosphatase 5
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DUSP5
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Reactivity Notes

Rat reported in scientific literature (PMID: 25397684).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DUSP5 Antibody (2F3)

• dual specificity phosphatase 5
• dual specificity protein phosphatase 5
• Dual specificity protein phosphatase hVH3
• DUSP
• EC 3.1.3.16
• EC 3.1.3.48
• HVH3VH1-like phosphatase 3
• serine/threonine specific protein phosphatase
• VH3

Background

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, is expressed in a variety of tissues with the highest levels in pancreas and brain, and is localized in the nucleus. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DUSP5 Antibody (H00001847-M04)(1)

Publication using H00001847-M04

• Fan Fan, Geurts Aron M, Pabbidi Mallikarjuna R et al. Zinc-finger nuclease knockout of dual-specificity protein phosphatase-5 enhances the myogenic response and autoregulation of cerebral blood flow in FHH.1BN rats. PLoS One. 2014-11-14 [PMID: 25397684] (WB, Rat)

Bioinformatics

• Gene Symbol: DUSP5
• Entrez:
  • 1847(Human)
• OMIM:
  • 603069(Human)
• Uniprot:
  • NP_004410(Human)