USP46 Antibody (CL0363) - Azide and BSA Free

Lane 1: Marker [kDa]Lane 2: Human cell line U-251 MG

Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-USP46 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Staining of U-2 OS cells using the anti-USP46 monoclonal antibody) .

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: CL0363
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

USP46 Antibody (CL0363) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse USP46 Antibody (CL0363) - Azide and BSA Free (NBP2-52923) is a monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of P62068, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: USP46
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Western Blot 1 ug/ml
• Application Notes: Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for USP46 Antibody (CL0363) - Azide and BSA Free

• Deubiquitinating enzyme 46
• EC 3.1.2.15
• EC 3.4.19.12
• FLJ11850
• FLJ12552
• FLJ14283
• FLJ39393
• ubiquitin carboxyl-terminal hydrolase 46
• ubiquitin specific peptidase 46
• ubiquitin specific protease 46
• ubiquitin thioesterase 46
• Ubiquitin thiolesterase 46
• Ubiquitin-specific-processing protease 46
• USP46

Background

Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinatedaction of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP46 belongs to a large family of cysteineproteases that function as deubiquitinating enzymes (Quesada et al., 2004 (PubMed 14715245)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: USP46