USP36 Antibody - BSA Free

Western Blot: USP36 Antibody [NB100-40832] - Whole cell lysate (50 ug) from HeLa, Hep-G2, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP36/DUB1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Immunohistochemistry-Paraffin: USP36 Antibody [NB100-40832] - Section of human colon carcinoma. Antibody: Affinity purified rabbit anti- USP36/DUB1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB

Immunoprecipitation: USP36 Antibody [NB100-40832] - Detection of Human USP36/DUB1 on HeLa whole cell lysate using NB100-40832. USP36/DUB1 was also immunoprecipitated by another rabbit anti- USP36/DUB1 antibody and NB100-40831.

Immunoprecipitation: USP36 Antibody [NB100-40832] - Detection of human USP36/DUB1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP36/DUB1 antibody NB100-40832 used for IP at 6 ug per reaction. USP36/DUB1 was also immunoprecipitated by another rabbit anti-USP36/DUB1 antibody. For blotting immunoprecipitated USP36/DUB1, NB100-40832 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

USP36 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit USP36 Antibody - BSA Free (NB100-40832) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-USP36 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1070 and the C-terminus (residue 1121) of human Ubiquitin Specific Peptidase 36 (Deubiquinating Enzyme 1) using the numbering given in entry NP_079366.2 (GeneID 57602).
• Marker: Nucleolus Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: USP36
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.2-4ug/ml
  • Immunohistochemistry-Paraffin 0.2-4ug/ml
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 0.4-1ug/ml
• Application Notes: Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
• Control:

  USP36 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for USP36 Antibody - BSA Free

• Deubiquitinating enzyme 36
• DUB1
• EC 3.1.2.15
• EC 3.4.19.12
• FLJ12851
• KIAA1453
• KIAA1453deubiquitinating enzyme 1
• ubiquitin carboxyl-terminal hydrolase 36
• ubiquitin specific peptidase 36
• ubiquitin specific protease 36
• ubiquitin thioesterase 36
• Ubiquitin thiolesterase 36
• Ubiquitin-specific-processing protease 36
• USP36

Background

USP36 belongs to the peptidase C19 family and is a deubiquitinating enzyme. USP36 contains the PEST motif and is polyubiquitinated. USP36 is broadly expressed. There are two named isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for USP36 Antibody (NB100-40832)(1)

Publication using NB100-40832

• Qiu W, Cai X, Xu K et al. PRL1 Promotes Glioblastoma Invasion and Tumorigenesis via Activating USP36-Mediated Snail2 Deubiquitination Frontiers in Oncology 2022-01-17 [PMID: 35111679] (Western Blot)

Bioinformatics

• Gene Symbol: USP36
• Entrez:
  • 57602(Human)
• Uniprot:
  • NP_079366.2(Human)