USP19 Antibody - Azide and BSA Free

Western Blot: USP19 Antibody [NBP2-93529] - Analysis of extracts of various cell lines, using USP19 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.

Immunocytochemistry/ Immunofluorescence: USP19 Antibody - Azide and BSA Free [NBP2-93529] - Immunofluorescence analysis of L929 cells using USP19 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

USP19 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit USP19 Antibody - Azide and BSA Free (NBP2-93529) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human USP19 (NP_001186089.1). MSGGASATGPRRGPPGLEDTTSKKKQKDRANQESKDGDPRKETGSRYVAQAGLEPLASGDPSASASHAAGITGSRHRTRLFFPSSSGSASTPQEEQTKEGACEDPHDLLATPTPELLLDWRQSAEEVIVKLRVGVGPLQLEDVDAAFTDTDCVVRFAGGQQWGGVFYAEIKSSCAKVQTRKGSLLHLTLPKKVPMLTWPS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: USP19
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for USP19 Antibody - Azide and BSA Free

• Deubiquitinating enzyme 19
• EC 3.1.2.15
• EC 3.4.19.12
• KIAA0891
• Ubiquitin Carboxyl-Terminal Hydrolase 19
• ubiquitin specific peptidase 19
• ubiquitin thioesterase 19
• Ubiquitin Thiolesterase 19
• Ubiquitin-specific-processing protease 19
• USP19
• Zinc finger MYND domain-containing protein 9
• ZMYND9
• ZMYND9ubiquitin specific protease 19

Background

Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 19 (USP19) is a member of this family. USP19 is a 146 kDa protein that contains two CS domains and one MYND-type zinc finger.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: USP19