Recombinant Human His6-USP1/His6-UAF1 Complex Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human His6-USP1/His6-UAF1 Complex Protein, CF Summary

Details of Functionality
Recombinant Human USP1/UAF1 complex is a Ubiquitin-specific deconjugating enzyme heterodimer. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP1/UAF1 complex concentration of 0.01-0.5 μM.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP1/UAF1 Complex protein
Contains a C-terminal 6-His tag
Accession #
Protein/Peptide Type
Recombinant Enzymes
Gene
USP1
Purity
>80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

Theoretical MW
89 kDa (USP1), 77 kDa (UAF1).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
E-568 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer

X mg/ml total protein including USP1 (X µM) and UAF1(X µM) in 50 mM HEPES, pH 7.5, 100 mM NaCl, 10% glycerol, 1 mM TCEP

Purity
>80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-USP1/His6-UAF1 Complex Protein, CF

  • USP1/UAF1 Complex

Background

USP1 (Ubiquitin-Specific-processing Protease 1, also known as Ubiquitin carboxyl-terminal hydrolase 1) is a deubiquitinating enzyme of the C19 peptidase family and functions as a negative regulator of the Fanconi Anemia pathway. Reported substrates of USP1 include monoubiquitinated FANCD2 and monoubiquitinated PCNA. USP1 plays important roles in DNA damage responses and cancer-related processes, and inhibiting the function of this deubiquitinase sensitizes some cancer cells to chemotherapy. By itself, USP1 is nearly completely inactive and requires a protein binding partner, UAF1 (USP1-Associated Factor 1, also known as WD Repeat-containing protein 48 or WDR48) to stimulate its deubiquitinase activity. In addition to the naturally occurring substates listed above, the USP1/UAF1 complex has been demonstrated to cleave K6, K33, and K63-linked polyubiquitin chains. Recombinant USP1 protein contains a C-terminal 6-His tag, and glycine-to-alanine substitutions at positions 670 and 671 (UniProt O94782 numbering). Recombinant UAF1 protein also contains a C-terminal 6-His tag. The assembled complex contains 25 µg each of USP1 and UAF1, resulting in a 1:1.2 stoichiometry.

  1. Cohn M.A., et al. (2009) J Biol. Chem. 284: 5343-5351
  2. Huang T.T., et al. (2006) Nat. Cell Biol. 8: 339-347
  3. Joo H-Y., et al. (2011) Biol. Chem. 286: 7190-7201
  4. Moretti J., et al. (2012) J Biol. Chem. 287: 29429-29441
  5. Williams S.A, et al. (2011)  Cell 146: 918–930

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Publications for USP1/UAF1 Complex (E-568)(4)

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Bioinformatics

Gene Symbol USP1
Uniprot