Recombinant Human His6-USP1/His6-UAF1 Complex Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human His6-USP1/His6-UAF1 Complex Protein, CF Summary

• Details of Functionality: Recombinant Human USP1/UAF1 complex is a Ubiquitin-specific deconjugating enzyme heterodimer. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP1/UAF1 complex concentration of 0.01-0.5 μM.
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP1/UAF1 Complex protein
  Met1 - Leu785 (Gly670Ala, Gly671Ala) with a C-terminal 6-His tag (USP1), Met1 - Thr677 with a C-terminal 6-His tag (UAF1)
• Accession #: O94782.1 (USP1), Q8TAF3.1 (UAF1)
• Protein/Peptide Type: Recombinant Enzymes
• Gene: USP1

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 89 kDa (USP1), 77 kDa (UAF1).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: Supplied as a 0.2 μm filtered solution in HEPES, NaCl, TCEP and Glycerol.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-USP1/His6-UAF1 Complex Protein, CF

• USP1/UAF1 Complex

Background

USP1 (Ubiquitin-Specific-processing Protease 1, also known as Ubiquitin carboxyl-terminal hydrolase 1) is a deubiquitinating enzyme of the C19 peptidase family and functions as a negative regulator of the Fanconi Anemia pathway. Reported substrates of USP1 include monoubiquitinated FANCD2 and monoubiquitinated PCNA. USP1 plays important roles in DNA damage responses and cancer-related processes, and inhibiting the function of this deubiquitinase sensitizes some cancer cells to chemotherapy. Alone, USP1 is nearly completely inactive and requires a protein binding partner, UAF1 (USP1-Associated Factor 1, also known as WD Repeat-containing protein 48 or WDR48) to stimulate its deubiquitinase activity. In addition to the naturally occurring substates listed above, the USP1/UAF1 complex has been demonstrated to cleave K6, K33, and K63-linked polyubiquitin chains. 

1. Cohn M.A., et al. (2009) J. Biol. Chem. 284: 5343.
2. Huang T.T., et al. (2006) Nat. Cell Biol. 8: 339.
3. Joo H-Y., et al. (2011) Biol. Chem. 286: 7190.
4. Moretti J., et al. (2012) J. Biol. Chem. 287: 29429.
5. Williams S.A, et al. (2011)  Cell 146: 918.

Publications for USP1/UAF1 Complex (E-568)(5)

Publications using E-568

• A Tyagi, K Kaushal, AP Chandrasek, N Sarodaya, S Das, CH Park, SH Hong, KS Kim, S Ramakrishn CRISPR/Cas9-based genome-wide screening for deubiquitinase subfamily identifies USP1 regulating MAST1-driven cisplatin-resistance in cancer cells Theranostics, 2022;12(13):5949-5970. 2022 [PMID: 35966591] (Bioassay, Human)
• AP Lawson, DW Bak, DA Shannon, MJC Long, T Vijaykumar, R Yu, FE Oualid, E Weerapana, L Hedstrom Identification of deubiquitinase targets of isothiocyanates using SILAC-assisted quantitative mass spectrometry Oncotarget, 2017;8(31):51296-51316. 2017 [PMID: 28881649] (Bioassay, Human)
• AP Lawson, DW Bak, DA Shannon, MJC Long, T Vijaykumar, R Yu, FE Oualid, E Weerapana, L Hedstrom Identification of deubiquitinase targets of isothiocyanates using SILAC-assisted quantitative mass spectrometry Oncotarget, 2017;8(31):51296-51316. 2017 [PMID: 28881649] (Bioassay, Human)
• Sylvie van Twest Mechanism of Ubiquitination and Deubiquitination in the Fanconi Anemia Pathway Mol. Cell, 2016;0(0):. 2016 [PMID: 27986371] (Bioassay)
• E Lecona, S Rodriguez-, J Specks, AJ Lopez-Cont, I Ruppen, M Murga, J Muñoz, J Mendez, O Fernandez- USP7 is a SUMO deubiquitinase essential for DNA replication Nat Struct Mol Biol, 2016;23(4):270-7. 2016 [PMID: 26950370] (Bioassay, Human)

Bioinformatics

• Gene Symbol: USP1
• Uniprot:
  • O94782.1 (USP1), Q8TAF3.1 (UAF1)()