Recombinant Human His6-USP1/His6-UAF1 Complex Protein, CF Summary
Details of Functionality
Recombinant Human USP1/UAF1 complex is a Ubiquitin-specific deconjugating enzyme heterodimer. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP1/UAF1 complex concentration of 0.01-0.5 μM.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP1/UAF1 Complex protein Met1 - Leu785 (Gly670Ala, Gly671Ala) with a C-terminal 6-His tag (USP1), Met1 - Thr677 with a C-terminal 6-His tag (UAF1)
89 kDa (USP1), 77 kDa (UAF1). Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -70 °C as supplied.
3 months, -70 °C under sterile conditions after opening.
Supplied as a 0.2 μm filtered solution in HEPES, NaCl, TCEP and Glycerol.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human His6-USP1/His6-UAF1 Complex Protein, CF
USP1 (Ubiquitin-Specific-processing Protease 1, also known as Ubiquitin carboxyl-terminal hydrolase 1) is a deubiquitinating enzyme of the C19 peptidase family and functions as a negative regulator of the Fanconi Anemia pathway. Reported substrates of USP1 include monoubiquitinated FANCD2 and monoubiquitinated PCNA. USP1 plays important roles in DNA damage responses and cancer-related processes, and inhibiting the function of this deubiquitinase sensitizes some cancer cells to chemotherapy. Alone, USP1 is nearly completely inactive and requires a protein binding partner, UAF1 (USP1-Associated Factor 1, also known as WD Repeat-containing protein 48 or WDR48) to stimulate its deubiquitinase activity. In addition to the naturally occurring substates listed above, the USP1/UAF1 complex has been demonstrated to cleave K6, K33, and K63-linked polyubiquitin chains.
Cohn M.A., et al. (2009) J. Biol. Chem. 284: 5343.
Huang T.T., et al. (2006) Nat. Cell Biol. 8: 339.
Joo H-Y., et al. (2011) Biol. Chem. 286: 7190.
Moretti J., et al. (2012) J. Biol. Chem. 287: 29429.
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