Recombinant Human USP15 Protein

Product Discontinued

Recombinant Human USP15 Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-235 of Human USP15
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAEGGAA DLDTQRSDIA TLLKTSLRKG DTWYLVDSRW FKQWKKYVGF DSWDKYQMGD QNVYPGPIDN SGLLKDGDAQ SLKEHLIDEL DYILLPTEGW NKLVSWYTLM EGQEPIARKV VEQGMFVKHC KVEVYLTELK LCENGNMNNV VTRRFSKADT IDTIEKEIRK IFSIPDEKET RLWNKYMSNT FEPLNKPDST IQDAGLYQGQ VLVIEQKNED GTWPRGPSTP KKPLEQSC

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: USP15
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 29.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 10% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human USP15 Protein

• Deubiquitinating enzyme 15
• EC 3.1.2.15
• EC 3.4.19.12
• Gcap18
• KIAA0529
• KIAA0529ubiquitin carboxyl-terminal hydrolase 15
• MGC131982
• MGC149838
• MGC74854
• ubiquitin specific peptidase 15
• ubiquitin specific protease 15
• ubiquitin thioesterase 15
• Ubiquitin thiolesterase 15
• Ubiquitin-specific-processing protease 15
• Ubp109
• UNPH-2
• UNPH4
• USP15

Background

USP15 is a member of the ubiquitin specific protease (USP) family of deubiquitinating enzymes. USP enzymes play critical roles in ubiquitin-dependent processes through polyubiquitin chain disassembly and hydrolysis of ubiquitin-substrate bonds. The protein associates with the COP9 signalosome, and also plays a role in transforming growth factor beta signalling through deubiquitination of receptor-activated SMAD transcription factors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 2. Recombinant human USP15 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: USP15