USP14 Antibody

Images

 
Western Blot: USP14 Antibody [NBP2-20826] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:5000.
Immunohistochemistry-Paraffin: USP14 Antibody [NBP2-20826] - Immunohistochemical analysis of paraffin-embedded Normal Liver, using antibody at 1:250 dilution.
Western Blot: USP14 Antibody [NBP2-20826] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

USP14 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human USP14. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
USP14
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (100%), Chimpanzee (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for USP14 Antibody

  • Deubiquitinating enzyme 14
  • EC 3.1.2.15
  • EC 3.4.19.12
  • TGT
  • TGTubiquitin carboxyl-terminal hydrolase 14
  • tRNA-guanine transglycosylase, 60-kD subunit
  • ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
  • ubiquitin specific protease 14 (tRNA-guanine transglycosylase)
  • Ubiquitin Thioesterase 14
  • Ubiquitin thiolesterase 14
  • ubiquitin-specific processing protease 14
  • Ubiquitin-specific-processing protease 14
  • Ubp6
  • USP14

Background

This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol USP14
Entrez
Uniprot