![Western Blot: USP13 Antibody [NBP1-46203] - Detection of human and mouse USP13 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, and mouse TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP13 antibody NBP1-46203 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/USP13-Antibody-Western-Blot-NBP1-46203-img0009.jpg "Western Blot: USP13 Antibody [NBP1-46203] - Detection of human and mouse USP13 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, and mouse TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP13 antibody NBP1-46203 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
| Immunogen | The immunogen for this product maps to a region between residue 175 and 225 of human Ubiquitin Specific Peptidase 13 using the numbering given in entry NP_003931.2 (GeneID 8975). |
| Predicted Species | Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | USP13 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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Beclin 1: Regulator of Autophagy and Apoptosis Beclin 1 is the mammalian orthologue of the yeast Apg6/Vps30 gene. Beclin 1 can complement the defect in autophagy present in apg6 yeast strains and stimulate autophagy when overexpressed in mammalian cells (1) and can bind to Bcl2, an important regul... Read full blog post. |
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| Gene Symbol | USP13 |
![Immunohistochemistry-Paraffin: USP13 Antibody [NBP1-46203] - Section of human colon carcinoma. Antibody: Affinity purified rabbit anti-USP13 (NBP1-46203). Detection: DAB](http://images.novusbio.com/fullsize/USP13-Antibody-Immunohistochemistry-NBP1-46203-img0006.jpg "Immunohistochemistry-Paraffin: USP13 Antibody [NBP1-46203] - Section of human colon carcinoma. Antibody: Affinity purified rabbit anti-USP13 (NBP1-46203). Detection: DAB")
![Immunohistochemistry-Paraffin: USP13 Antibody [NBP1-46203] - Detection of mouse USP13 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-USP13 (NBP1-46203). Detection: DAB](http://images.novusbio.com/fullsize/USP13-Antibody-Immunohistochemistry-Paraffin-NBP1-46203-img0007.jpg "Immunohistochemistry-Paraffin: USP13 Antibody [NBP1-46203] - Detection of mouse USP13 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-USP13 (NBP1-46203). Detection: DAB")
![Immunoprecipitation: USP13 Antibody [NBP1-46203] - Detection of human USP13 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP13 antibody NBP1-46203 used for IP at 3 ug per reaction. USP13 was also immunoprecipitated by another rabbit anti-USP13 antibody. For blotting immunoprecipitated USP13, NBP1-46203 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/USP13-Antibody-Immunoprecipitation-NBP1-46203-img0008.jpg "Immunoprecipitation: USP13 Antibody [NBP1-46203] - Detection of human USP13 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP13 antibody NBP1-46203 used for IP at 3 ug per reaction. USP13 was also immunoprecipitated by another rabbit anti-USP13 antibody. For blotting immunoprecipitated USP13, NBP1-46203 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![ISG15/UCRP [7-Amino-4-Methyl Coumarin]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "ISG15/UCRP [7-Amino-4-Methyl Coumarin]")
