Recombinant Human USP2 Catalytic Domain Protein, CF

• Reactivity: Hu
• Applications: Enzyme Activity
• Format: Carrier-Free

Recombinant Human USP2 Catalytic Domain Protein, CF Summary

• Details of Functionality: Recombinant Human USP2 Catalytic Domain is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP2 Catalytic Domain concentration of 1-5 nM.
• Source: E. coli-derived human USP2 protein
  Asn259 - Met605
• Accession #: O75604.2
• Protein/Peptide Type: Recombinant Enzymes
• Gene: USP2
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: Supplied as a solution in HEPES, NaCl, EDTA and DTT.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human USP2 Catalytic Domain Protein, CF

• Deubiquitinating enzyme 2
• EC 3.1.2.15
• EC 3.4.19.12,41 kDa ubiquitin-specific protease
• ubiquitin specific peptidase 2
• ubiquitin specific protease 12
• ubiquitin specific protease 2
• ubiquitin specific protease 9
• ubiquitin thioesterase 2
• Ubiquitin thiolesterase 2
• Ubiquitin-specific-processing protease 2
• UBP41
• UBP41ubiquitin carboxyl-terminal hydrolase 2
• USP2
• USP9

Background

Ubiquitin Specific Peptidase 2 (USP2), also known as UBP41 and USP9, is a cytoplasmic protein that belongs to the peptidase C19 family of deubiquitinating enzymes (1). It is widely expressed and serves to deubiquitinate select target substrates. At least three isoforms have thus far been described: USP2a, also known as USP2-69, USP-41, and USP2-45 (2,3). All isoforms contain the same catalytic core, but show variations within their N- and C-terminal domains (3). USP2a is the canonical isoform. It is 605 amino acids (aa) in length and has a predicted molecular weight of 68 kDa. Human USP2a shares 90% aa sequence identity with the mouse and rat orthologs. USP-41 and USP-45 are 362 and 396 aa in length, respectively, and have predicted molecular weights of 41 and 45 kDa. USP-41 and USP2a have been shown to be critical regulators of apoptosis and cell proliferation. They deubiquitinate poly-ubiquitinated target proteins, such as FAS, MDM2, and Cyclin D1, to promote or block apoptosis, depending on the context (3-9). USP-45, whose expression appears to be regulated by circadian and nutritional cues, is thought to regulate hepatic gluconeogenesis and glucose metabolism (10,11). This recombinant human protein encompasses the catalytic domain of USP2 (aa 259-605).

1. Baek, S.H. et al. (1997) J. Biol. Chem. 272:25560.
2. Gousseva, N. & R.T. Baker (2003) Gene Expr. 11:163.
3. Haimerl, F. et al. (2009) J. Biol. Chem. 284:495.
4. Gewies, A. & S. Grimm (2003) Cancer Res. 63:682.
5. Graner, E. et al. (2004) Cancer Cell 5:253.
6. Stevenson, L.F. et al. (2007) EMBO J. 26:976.
7. Shan, J. et al. (2009) Mol. Cell 36:469.
8. Mahul-Mellier, A.L. et al. (2012) Cell Death Differ. 19:891.
9. Mahul-Mellier, A.L. et al. (2012) Biochim. Biophys. Acta. 1823:1353.
10. Molusky, M.M. et al. (2012) Diabetes 61:1025.
11. Molusky, M.M. et al. (2012) PLoS One 7:e47970.

Publications for USP2 (E-504)(29)

Publications using E-504

• Mukhopadhyay, U;Levantovsky, S;Carusone, TM;Gharbi, S;Stein, F;Behrends, C;Bhogaraju, S; A ubiquitin-specific, proximity-based labeling approach for the identification of ubiquitin ligase substrates Science advances 2024-08-09 [PMID: 39121224] (Bioassay, N/A)
• Pereira, M;Ramalho, T;Andrade, WA;Durso, DF;Souza, MC;Fitzgerald, KA;Golenbock, DT;Silverman, N;Gazzinelli, RT; The IRAK1/IRF5 axis initiates IL-12 response by dendritic cells and control of Toxoplasma gondii infection Cell reports 2024-02-15 [PMID: 38367238] (Bioassay, Mouse)
• Isberg, R;Kotewicz, K;Zheng, M;Kim, S;Tai, A; Sde Proteins Coordinate Ubiquitin Utilization and Phosphoribosylation to Promote Establishment and Maintenance of the Legionella Replication Vacuole Research square 2023-09-18 [PMID: 37790456] (Bioassay, N/A)
• OV Kochenova, S Mukkavalli, M Raman, JC Walter Cooperative assembly of p97 complexes involved in replication termination Nature Communications, 2022-11-03;13(1):6591. 2022-11-03 [PMID: 36329031] (Bioassay, Human)
• X Wang, F Zheng, YY Yi, GY Wang, LX Hong, D McCollum, C Fu, Y Wang, QW Jin Ubiquitination of CLIP-170 family protein restrains polarized growth upon DNA replication stress Nature Communications, 2022-09-22;13(1):5565. 2022-09-22 [PMID: 36138017] (Bioassay, Human)
• T Pham, AJ Najy, HC Kim E3 ligase HUWE1 promotes PDGF D-mediated osteoblastic differentiation of mesenchymal stem cells by effecting polyubiquitination of beta-PDGFR The Journal of Biological Chemistry, 2022-04-25;0(0):101981. 2022-04-25 [PMID: 35472332] (Bioassay, Human)
• RS Marshall, RD Vierstra A trio of ubiquitin ligases sequentially drives ubiquitylation and autophagic degradation of dysfunctional yeast proteasomes Cell Reports, 2022-03-15;38(11):110535. 2022-03-15 [PMID: 35294869] (Bioassay)
• H Zhou, MS Zaher, JC Walter, A Brown Structure of CRL2Lrr1, the E3 ubiquitin ligase that promotes DNA replication termination in vertebrates Nucleic Acids Research, 2021-12-16;49(22):13194-13206. 2021-12-16 [PMID: 34850944] (Bioassay, N/A)
• EG Otten, E Werner, A Crespillo-, KB Boyle, V Dharamdasa, C Pathe, B Santhanam, F Randow Ubiquitylation of lipopolysaccharide by RNF213 during bacterial infection Nature, 2021-05-19;0(0):. 2021-05-19 [PMID: 34012115] (Bioassay, Bacteria - Salmonella typhimurium)
• LD Harris, J Le Pen, N Scholz, J Mieszczane, N Vaughan, S Davis, G Berridge, BM Kessler, M Bienz, JDF Licchesi The deubiquitinase TRABID stabilizes the K29/K48-specific E3 ubiquitin ligase HECTD1 The Journal of Biological Chemistry, 2021-01-08;296(0):100246. 2021-01-08 [PMID: 33853758] (Bioassay, Human)
• MJ McBride, N Mashtalir, EB Winter, HT Dao, M Filipovski, AR D'Avino, HS Seo, NT Umbreit, R St Pierre, AM Valencia, K Qian, HJ Zullow, JD Jaffe, S Dhe-Pagano, TW Muir, C Kadoch The nucleosome acidic patch and H2A ubiquitination underlie mSWI/SNF recruitment in synovial sarcoma Nat. Struct. Mol. Biol., 2020-08-03;0(0):. 2020-08-03 [PMID: 32747783] (Bioassay, Human)
• Y Isobe, M Okumura, LM McGregor, SM Brittain, MD Jones, X Liang, R White, W Forrester, JM McKenna, JA Tallarico, M Schirle, TJ Maimone, DK Nomura Manumycin polyketides act as molecular glues between UBR7 and P53 Nat. Chem. Biol., 2020-06-22;0(0):. 2020-06-22 [PMID: 32572277] (Bioassay, Human)
• MN Okur, JH Lee, W Osmani, R Kimura, TG Demarest, DL Croteau, VA Bohr Cockayne syndrome group A and B proteins function in rRNA transcription through nucleolin regulation Nucleic Acids Res., 2020-03-18;0(0):. 2020-03-18 [PMID: 31970402] (Bioassay, Human)
• M Fottner, AD Brunner, V Bittl, D Horn-Ghetk, A Jussupow, VRI Kaila, A Bremm, K Lang Site-specific ubiquitylation and SUMOylation using genetic-code expansion and sortase Nat. Chem. Biol., 2019-02-15;15(3):276-284. 2019-02-15 [PMID: 30770915] (Ubiquitination, Human)
• Y Liang, WJ Dearnaley, NA Alden, MJ Solares, BL Gilmore, KJ Pridham, AC Varano, Z Sheng, E Alli, DF Kelly Correcting errors in the BRCA1 warning system DNA Repair (Amst.), 2018-11-22;73(0):120-128. 2018-11-22 [PMID: 30503669] (Bioassay)
• P Tonzi, Y Yin, CWT Lee, E Rothenberg, TT Huang Translesion polymerase kappa-dependent DNA synthesis underlies replication fork recovery Elife, 2018-11-13;7(0):. 2018-11-13 [PMID: 30422114] (Bioassay, Human)
• QA Khan, P Pediaditak, Y Malakhau, A Esmaeilnia, Z Ashkavand, V Sereda, NI Krupenko, SA Krupenko CHIP E3 ligase mediates proteasomal degradation of the proliferation regulatory protein ALDH1L1 during the transition of NIH3T3 fibroblasts from G0/G1 to S-phase PLoS ONE, 2018-07-06;13(7):e0199699. 2018-07-06 [PMID: 29979702] (Bioassay, Human)
• JR Blount, K Libohova, GB Marsh, JR Sutton, SV Todi Expression and Regulation of Deubiquitinase-Resistant, Unanchored Ubiquitin Chains in Drosophila Sci Rep, 2018-05-31;8(1):8513. 2018-05-31 [PMID: 29855490] (Bioassay)
• SR Nath, Z Yu, TA Gipson, GB Marsh, E Yoshidome, DM Robins, SV Todi, DE Housman, AP Lieberman Androgen receptor polyglutamine expansion drives age-dependent quality control defects and muscle dysfunction J. Clin. Invest., 2018-07-23;0(0):. 2018-07-23 [PMID: 29809168] (Bioassay)
• GL McLelland, T Goiran, W Yi, G Dorval, CX Chen, ND Lauinger, AI Krahn, S Valimehr, A Rakovic, I Rouiller, TM Durcan, JF Trempe, EA Fon Mfn2 ubiquitination by PINK1/parkin gates the p97-dependent release of ER from mitochondria to drive mitophagy Elife, 2018-04-20;7(0):. 2018-04-20 [PMID: 29676259] (Bioassay, Human)
• Z Sha, HM Schnell, K Ruoff, A Goldberg Rapid induction of p62 and GABARAPL1 upon proteasome inhibition promotes survival before autophagy activation J. Cell Biol., 2018-03-13;0(0):. 2018-03-13 [PMID: 29535191] (Bioassay, Human)
• EY Huang, M To, E Tran, LTA Dionisio, HJ Cho, KLM Baney, CI Pataki, JA Olzmann A VCP inhibitor substrate trapping approach (VISTA) enables proteomic profiling of endogenous ERAD substrates Mol. Biol. Cell, 2018-03-22;0(0):. 2018-03-22 [PMID: 29514927] (Bioassay)
• X Liu, JM Reitsma, JL Mamrosh, Y Zhang, R Straube, RJ Deshaies Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein Expression Mol. Cell, 2018-03-01;69(5):773-786.e6. 2018-03-01 [PMID: 29499133] (Bioassay, Human)
• K Glaeser, M Urban, E Fenech, O Voloshanen, D Kranz, F Lari, JC Christians, M Boutros ERAD-dependent control of the Wnt secretory factor Evi EMBO J., 2018-01-29;37(4):. 2018-01-29 [PMID: 29378775] (Bioassay, Human)
• AS Murray, FA Varela, TE Hyland, AJ Schoenbeck, JM White, LM Tanabe, SV Todi, K List Phosphorylation of the type II transmembrane serine protease, TMPRSS13 in Hepatocyte Growth Factor Activator Inhibitor-1 and 2-mediated cell surface localization J. Biol. Chem., 2017-07-14;0(0):. 2017-07-14 [PMID: 28710277] (Bioassay, Human)
• Y Lee, TF Chou, SK Pittman, AL Keith, B Razani, CC Weihl Keap1/Cullin3 Modulates p62/SQSTM1 Activity via UBA Domain Ubiquitination Cell Rep, 2017-04-04;19(1):188-202. 2017-04-04 [PMID: 28380357] (Bioassay, Mouse)
• TV Nguyen, J Li, CJ Lu, JL Mamrosh, G Lu, BE Cathers, RJ Deshaies p97/VCP promotes degradation of CRBN substrate glutamine synthetase and neosubstrates Proc. Natl. Acad. Sci. U.S.A, 2017-03-20;0(0):. 2017-03-20 [PMID: 28320958] (Bioassay)
• HE Kamber Kay, M Ditzel, P Meier, A Bergmann An inhibitory mono-ubiquitylation of the Drosophila initiator caspase Dronc functions in both apoptotic and non-apoptotic pathways PLoS Genet, 2017-02-16;13(2):e1006438. 2017-02-16 [PMID: 28207763] (Bioassay, Drosophila)
• Liang , Qin, Dexheimer , Thomas S, Zhang , Ping, Rosenthal , Andrew S, Villamil , Mark A, You , Changjun, Zhang , Qiuting, Chen , Junjun, Ott , Christin, Sun , Hongmao, Luci , Diane K, Yuan , Bifeng, Simeonov , Anton, Jadhav , Ajit, Xiao , Hui, Wang , Yinsheng, Maloney , David J, Zhuang , Zhihao A selective USP1-UAF1 inhibitor links deubiquitination to DNA damage responses. Nat Chem Biol, 2014-02-16;10(4):298-304. 2014-02-16 [PMID: 24531842] (Bioassay)

Bioinformatics

• Gene Symbol: USP2
• Uniprot:
  • O75604.2()