USP10 Antibody

Western Blot: USP10 Antibody [NB110-40545] - Detection of mouse USP10 by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP10 antibody NB110-40545 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry-Paraffin: USP10 Antibody [NB110-40545] - Detection of human USP10 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- USP10 (NB110-40545). Detection: DAB

Western Blot: USP10 Antibody [NB110-40545] - Detection of Human USP10 on HeLa whole cell lysate using NB110-40545. USP10 was also immunoprecipitated by another rabbit anti-USP10 antibody and NB110-40546.

Western Blot: USP10 Antibody [NB110-40545] - Detection of human USP10 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP10 antibody NB110-40545 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry-Paraffin: USP10 Antibody [NB110-40545] - FFPE section of human lung adenocarcinoma. Affinity purified rabbit anti-USP10 used at a dilution of 1:100.

Immunohistochemistry: USP10 Antibody [NB110-40545] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- USP10 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

USP10 Antibody Summary

• Description: Novus Biologicals Rabbit USP10 Antibody (NB110-40545) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-USP10 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 50 and 100 of human Ubiquitin Specific Peptidase 10 using the numbering given in entry NP_005144.1 (GeneID 9100).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: USP10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot
• Control:

  USP10 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for USP10 Antibody

• Deubiquitinating enzyme 10
• EC 3.4.19.12
• KIAA0190MGC2621
• ubiquitin carboxyl-terminal hydrolase 10
• ubiquitin specific peptidase 10
• ubiquitin specific protease 10
• ubiquitin thioesterase 10
• Ubiquitin Thiolesterase 10
• Ubiquitin-specific-processing protease 10
• UBPO
• Uchrp
• USP10

Background

Ubiquitin is a highly conserved protein that is covalently linked to other proteins to regulate their function and degradation. This gene encodes a member of the ubiquitin-specific protease family of cysteine proteases. The enzyme specifically cleaves ubiquitin from ubiquitin-conjugated protein substrates. The protein is found in the nucleus and cytoplasm. It functions as a co-factor of the DNA-bound androgen receptor complex, and is inhibited by a protein in the Ras-GTPase pathway. The human genome contains several pseudogenes similar to this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for USP10 Antibody (NB110-40545)(2)

Publications using NB110-40545

• Koeppen K, Chapline C, Sato JD et al. Nedd4-2 Does Not Regulate wt-CFTR in Human Airway Epithelial Cells. Am J Physiol Lung Cell Mol Physiol 2012-08-23 [PMID: 22904170]
• Simpson-Holley M, Kedersha N, Dower K et al. Formation of antiviral cytoplasmic granules during orthopoxvirus infection. J Virol 2011-02-01 [PMID: 21147913]

Bioinformatics

• Gene Symbol: USP10
• Entrez:
  • 9100(Human)
• Uniprot:
  • NP_005144.1(Human)