Uromodulin Antibody

Western Blot: Uromodulin Antibody [H00007369-B01P] - Analysis of UMOD expression in transfected 293T cell line by UMOD polyclonal antibody. Lane 1: UMOD transfected lysate(67.21 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: Uromodulin Antibody [H00007369-B01P] - Analysis of purified antibody to UMOD on HeLa cell. (antibody concentration 10 ug/ml)

Immunohistochemistry-Paraffin: Uromodulin Antibody [H00007369-B01P] - Analysis of purified antibody to UMOD on formalin-fixed paraffin-embedded human kidney. (antibody concentration 3 ug/ml)

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated

Uromodulin Antibody Summary

• Immunogen: UMOD (AAH35975, 1 a.a. - 611 a.a.) full-length human protein. MGQPSLTWMLMVVVASWFITTAATDTSEARWCSECHSNATCTEDEAVTTCTCQEGFTGDGLTCVDLDECAIPGAHNCSANSSCVNTPGSFSCVCPEGFRLSPGLGCTDVDECAEPGLSHCHALATCVNVVGSYLCVCPAGYRGDGWHCECSPGSCGPGLDCVPEGDALVCADPCQAHRTLDEYWRSTEYGEGYACDTDLRGWYRPHPSSDEGIVSRKACAHWSGHCCLWDASVQVKACAGGYYVYNLTAPPECHLAYCTDPSSVEGTCEECSIDEDCKSNNGRWHCQCKQDFNITDISLLEHRLECGANDMKVSLGKCQLKSLGFDKVFMYLSDSRCSGFNDRDNRDWVSVVTPARDGPCGTVLTRNETHATYSNTLYLADEIIIRDLNIKINFACSYPLDMKVSLKTALQPMVSALNIRVGGTGMFTVRMALFQTPSYTQPYQGSSVTLSTEAFLYVGTMLDGGDLSRFALLMTNCYATPSSNATDPLKYFIIQDRCPHTRDSTIQVVENGESSQGRFSVQMFRFAGNYDLVYLHCEVYLCDTMNEKCKPTCSGTRFRSGSVIDQSRVLNLGPITRKGVQATVSRAFSSLGLLKVWLPLLLSATLTLTFQ
• Specificity: UMOD - uromodulin (uromucoid, Tamm-Horsfall glycoprotein),
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: UMOD
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. May also be used for IF, IHC-P. Use in FLOW reported in scientific literature (Santelli A et al).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Uromodulin Antibody

• ADMCKD2
• FJHN
• HNFJ
• HNFJ1
• MCKD2
• Tamm-Horsfall glycoprotein
• Tamm-Horsfall urinary glycoprotein
• THGP
• THP
• UMOD
• uromodulin (uromucoid, Tamm-Horsfall glycoprotein)
• Uromodulin
• Uromucoid

Background

This gene encodes uromodulin, the most abundant protein in normal urine. Its excretion in urine follows proteolytic cleavage of the ectodomain of its glycosyl phosphatidylinosital-anchored counterpart that is situated on the luminal cell surface of the loop of Henle. Uromodulin may act as a constitutive inhibitor of calcium crystallization in renal fluids. Excretion of uromodulin in urine may provide defense against urinary tract infections caused by uropathogenic bacteria. Defects in this gene are associated with the autosomal dominant renal disorders medullary cystic kidney disease-2 (MCKD2) and familial juvenile hyperuricemic nephropathy (FJHN). These disorders are characterized by juvenile onset of hyperuricemia, gout, and progressive renal failure. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same isoform. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Uromodulin Antibody (H00007369-B01P)(2)

Publications using H00007369-B01P

• Larkin JR, Zhang F, Godfrey L et al. Glucose-induced down regulation of thiamine transporters in the kidney proximal tubular epithelium produces thiamine insufficiency in diabetes. PLoS One. 2012 Dec 28 [PMID: 23285265]
• Santelli A, Sun I, Eirin A, et al. Senescent Kidney Cells in Hypertensive Patients Release Urinary Extracellular Vesicles JAHA 2019 Jul 01 (Flow, Human)

Bioinformatics

• Gene Symbol: UMOD
• Entrez:
  • 7369(Human)
• Uniprot:
  • AAH35975(Human)