Uromodulin Antibody (877914) [Alexa Fluor® 488]

• Applications: WB, IHC
• Clone: 877914
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Uromodulin Antibody (877914) [Alexa Fluor® 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Uromodulin
  Asp25-Ser614
  Accession # P07911
• Specificity: Detects human Uromodulin in ELISA.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Uromodulin (also Tamm-Horsfall glycoprotein or THP) is an 85‑95 kDa urinary glycoprotein. It is secreted by renal tubule epithelium, acts as a binding protein for IL‑1, TNF-alpha and C1q, activates resting monocytes and promotes neutrophil phagocytosis. Uromodulin forms high molecular weight oligomers that line the kidnay tubules. Human Uromodulin is GPI‑linked. Its proprecursor is 616 amino acids (aa) in length. It contains three EGF-like domains (aa 28‑149), a ZP domain that mediates oligomerization (aa 334‑589) and a cleavable C‑terminal propeptide (aa 615‑640). There are multiple splice variants. One shows a deletion of aa 67‑199, a second shows a nine aa substitution for aa 609‑640, a third shows a Pro substitution for aa 205‑234 and a fourth shows a 66 aa substitution for aa 613‑640. Over aa 25‑614, human Uromodulin is 78% aa identical to mouse Uromodulin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.